| The fruit fly Drosophila melanogaster is an attractive model system for studying olfaction, duo to the unique combination of the organism's features and techniques available for use with Drosophila. In order to reveal genes effect on Drosophila olfactory nerve system and to provide significant resources for studying their functions, we initiated a genetic screen for genes altering the olfactory map in Drosophila by means of SG18.1-Gal4/UAS system, and characterized Akap200 gene, isolated in this study. We analyzed nrg gene isolated by a modifier screen in S2 Drosophila cell and investigated the molecular mechanism of interaction nrg with Limk. The main contexts are briefly described below.1. we initiated a genetic screen for Drosophila olfactory genes by means of SG18.1-Gal4/UAS system: The result validates our screen as an rapid, effective approach for recovering genes controlling glomerular map patterning; From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes; These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling. To begin to understand the effects of the P{GS} elements on the development of the ALs, we classified the lines by the nature of the genes affected and their influence on external sensilla development: Genes involved in axon guidance and synaptogenesis; Genes involved in transcription; Genes involved in cytoskeleton remodeling; Genes of undefined...
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