EST Analysis Of Physcomitrella Patens In Cold Stress And Primarily Functional Research Of Its Relational Gene Cor166

Low temperature is considered one of the most important environmental factors limiting the growth, development, planting, crop productivity, photosynthetic rate, and distribution of plants. But some temperate zone plants can adapt the futurecoming low temperature by producing some chemical and physiological metabolin in end autumn and primary spring, thus improves the resistance to low temperature in winter. Indicating plants evolve a set of perfect mechanism to response for low temperature. A clear understanding of plant cold-induced mechanism is a significant breeding objective in agriculture for improving crop productivity, and much species can be planted in winter.Physcomitrella patens distributed in wide world is a new developing plant model system in recent years with advantages: enduring extremely condition; a short life cycle; easy to culture, genetic transformation and homologous recombination. It facilitates the understanding of gene function for its long haploidy generation.as its morphological, chemical and physiological changes resulted from gene mutation or knock out are easy to be identified.A two directional SSHcDNA library of cold acclimation about Physcomitrella patens gametophyte was constructed. Dot blot hybridization was prepared with 384 random positive clones from forward and reverse library separately by blotting on Hybond N~+ membrane. 240 and 180 clones from forward and reverse library were sequenced according to dot blot hybridization information; 232 ESTs in forward library and 162 ESTs in reverse library can be further analyzed separately. The sequence similarity analysis in GenBank by BLSTx and BLSTxn shows that 215 ESTs encode for proteins (92.7% of total 232), 17 ESTs had no matchs in 232 ESTs. 120 ESTs encode for functionally known proteins and 95 ESTs for hypothetical, putative and unknown proteins among 215 ESTs; and in reverse library, 134 ESTs encode for proteins (82.7% of total 162), 28 ESTs had no matchs. 104 ESTs encode for functionally known proteins and 30 ESTs for hypothetical, putative and unknown protein among 134 ESTs. These best matched protein sequences are from 32 species, 30.3% from Arabidopsis thaliana, 15.8% from Oryza sativa, 7.9% from Triticum aestivum, 1.3%from physcomitrella patens in forward library; but in reverse library, 19.8% from Arabidopsis thaliana, 12.7% from Oryza sativa, 11.6% from physcomitrella patens, 5.8% from Nicotiana tobacum. EST contig analysis shows that there are 126 nonredundant ESTs in forward library, out of 126 ESTs, 106 ESTs are...