Coronavirus Gene Vaccines Mediated By Attenuated Salmonella Typhimurium And Their Immunobiological Properties

Since a new coronavirus was identified as the etiological agent of Severe Acute Respiratory Syndrome (SARS), it has evoked much interest in the molecular biology and immunology of coronavirus. The coronavirus, with the largest genome among all RNA viruses, can cause diseases in many animal species and human. Human coronaviruses, strain 229E and OC43, cause about 10% ~ 30% of the common colds. Other coronaviruses that infect respiratory, enteric, and central nervous system (CNS) induce serious diseases in porcine, bovine and chicken, and cause important economic losses. However, the pathogenesis and immunobiological properties of coronavirus were not very clear so far.Infectious bronchitis virus (IBV) causes an acute and highly contagious infectious disease in chicken. Attenuated vaccines were commonly used against IBV, but due to its lower efficacy and its potential risk to regain virulence,IB has become one of the most difficult diseases to control.Mouse hepatitis virus (MHV) induces hepatic, encephalomyelitis, and CNS demyelination in mice. The CNS infection with MHV provides an animal model for acute viral-induced encephalomyelitis and the human demyelinating disease- multiple sclerosis.In this study, the cDNA of S1 and N genes from IBV and MHV were amplified by RT-PCR, and recombinant eukaryotic plasmids expressing such S1 and N gene were constructed. Attenuated Salmonella typhimurium were used as a vector to deliver these...