Identification And Functional Characterization Of A Novel Gene MGC13096

There are only two isoforms of PDCD2 and MGC 13096 containing PDCD2_C domain in human genome. To study the role of PDCD2_C domain in apoptosis, the cDNAs of two isoforms of PDCD2 and MGC 13096 were cloned. The RT-PCR products (AY948416, AY948417) of PDCD2 from RNA of human embryonic kidney 293T (HEK293T) and gastric cancer AGS cell line lost common 99bp when compared with the sequences of NCBI database (NM 002598. NM 144781). The data of expression of PDCD2 and MGC 13096 genes in HEK293T cells which induced to undergo apoptosis by various treatments suggested that there wasn't significant over-regulation of MGC 13096 gene and the over-expression of PDCD2 gene was not occurred universally. Perhaps PDCD2_C domain is not universally associated with apoptosis. GEO (Gene Expression Omnibus) search about MGC13096 (Hs.515344) and GDS161 record program indicated MGC13096 was significantly up-regulation on muscle of insulin-resistant patients compared with that of insulin-sensitive people (GDS161 record | GPL100 RC_T92180_at). DNA/flow cytometry analysis showed that MGC13096 overexpression would delay the cell cycle progression at S phase severely. On the other hand, although its containing PDCD2_C domain (Programmed cell death protein 2, C-terminal putative domain),...