Study On Gene Silencing Of FRO1 And IRT Induced By Tomato Yellow Leaf Curl China Virus Viral Satellite DNA In Tomato Root

In plants, a procedure known as virus induced gene silencing (VIGS) has recently emerged as a powerful method for studying gene function. It was induced by infecting a plant with a plant virus that has had its genome modified to include a sequence identical to that in RNA transcribed from the host gene to be silenced. It will have appear gene silencing phenomenon in correspond to infection part of plant. It was aroused by RNA mediate plant resist disease defence and a correlation post-transcription gene silencing mechanism. With the great progress of mass determination of full-length sequence and expressed sequence tags (EST) in plant, VIGS provide a powerful tool to study the function of the plant genomic sequence.Up to today, several plant virus including RNA virus, DNA virus and satellite RNA virus were modified as a VIGS vector to study genomic function. Here we constructed a satellite DNA silencing vector (DNAmβ) by replacing βC1 gene with a multiple cloning site in TYLCCNV-Y10 DNAβ. Insertion into DNAmp of sequences from any of three host genes (PCNA, PDS, Su), or from a transgene (GFP), resulted in strong silencing of the cognate gene. Although DNAmβ probably does not enter meristematic tissue, the PCNA gene could be silenced there. But these gene silencing symptom is upside of the plant, tend to observe and identification. For the gene is especially expression in root, weather or not this vector can server as same efficacy. Therefore we deploy the study on gene silencing of Ferric Chelate Reductase and iron...