Interaction Of The Biomembranous Molecule And The Chemicals And The Magnetic Field

First of all, the concept, structure, composition and model of biomembrane has been presented, and the method has been expounded by researching biomembrane. Then, the influence of the chemical materials of five classes on the liquid crystal structure of biomembrane has been studied by the technique of modern physics. The interaction between unsaturated fatty acid and liposome, the anticancer of targeted liposomes, and the influence of the magnet field on the microcosmic structure of the surface of cancer cell membrane has been investigated respectively. Some anticancer platinum complexes have been synthesized on what has already been achieved. Their structure, physical and chemical character, and anticancer activation have been researched. The synergistic effect between the magnetic field and the anticancer platinum complexes has been investigated. It is of help on understanding biologic metabolism and prevention treatment of disease that a study has been made on the synergistic effect of the influence of the magnetic field and drug on the liquid crystal structure of biomembrane. It will bring forth the new ideas in the research work. It is of importantly worth in the science and latent applied prospects.The conformation of the liquid crystalline state in the interaction between chemical materials and biomembrane has been studied by the technique of modern physics. The experiments have proved that the biomembrane in the liquid crystalline state can be changed from the double layer phase into the non-double layer phase due to the influence of the outside environment. Besides the common cubic and hexagonal phases, some more other complex middle phases are also included in these non-double layer structures, namely the lamellar-cubic structures, the lamellar-hexagonal structures and the cubic-hexagonal structures. It is found that chemical materials can make the biomembrane in the liquid crystalline state assume non-double layer structures. Their ionic radius, the van der Waals and electrostatic interaction between the amphiphilic molecules, the electric dipole moment and pHvalue all have some effects on the liquid crystalline structure of biomembrane.The liquid crystal structure of the liposomes containing unsaturated fatty acid has been researched by means of small angle X-ray scattering (SAXS), 3IP nuclear magnetic resonance (31P-NMR) and scanning tunneling microscope (STM). The experiments have proved that there is the clearly influence of cholesterol, unsaturated fatty acid, non-ion activated agent on the liquid crystal structure of phosphatidylethanolamine (PE) liposomes. That the oleic heterogeneous liposomes and the linoleic heterogeneous liposomes are composed of the lamellar-cubic structure and the lamellar-hexagonal structure have been prepared in containing the efficient dispersing agent and the phosphate buffer, and the ricinoleic heterogeneous liposomes are composed of the cubic-hexagonal structure.The interaction between the liposomes containing unsaturated fatty acid and cancer cell membrane has been investigated by the technique of electron spin resonance (ESR), and inquired into that they have maybe biological significance in damaged and inhibited cancer cells. Experimental results showed following: the effect of the oleic heterogeneous liposomes causes that the powerful fixed effect of the spin probe is weakened and that the weak fixed effect of the spin probe is strengthened, and that the free direction of the spin probe movement is increased. The effect of linoleic heterogeneous liposomes cause that the powerful fixed effect of the spin probe is strengthened, and weakened on the membrane of mammary tumor, and that the free direction of the spin probe movement is impeded. The effect of ricinoleic heterogeneous liposomes is same with linoleic heterogeneous liposomes. The result indicates that the unsaturated fatty acid can react with mercaptan groups and change the conformations of membrane proteins of tumor cells.Linoleic platinum-containing untargeted and targeted liposomes bearing antihuman Breast cancer cell monoclonal antibody Me Ab Gp-1D8 on their surface are prepared by ultrasonic. The tissue distribution of linoleic platinum encapsulated liposomes and it efficacy of the tumor inhibit after injecting into the tumor-bearingnude mice as compared with that of free linoleic platinum were investigated. The result indicated that the content of linoleic platinum of targeted liposomes in the tumor tissues was apparently higher than that of free linoleic platinum group. However, the content of the former was lower than the latter in heart, liver, lung, spleen and kidney. Free linoleic platinum or targeted liposomes were injected into nude mice (4mg/Kg, body weight) at day 6, 12, and 24, respectively, and the data were obtained at 40 day after inoculation of breast cell. The result shows that linoleic platinum targeted liposomes has advantage over free linoleic platinum in some respects such as the amount of survived animals the ratio of tumor occurrence and the rate of tumor growth. The interaction of linoleic platinum targeted liposome with tumor cell membranes has been investigated by method of MSL spin Labeling. In the presence of linoleic platinum the ratio W/S of breast cancer cell membranes and S,g0 cell membranes labeled with MLS were changed. The result indicates that linoleic platinum can react with mercaptan groups and change the conformations of membrane proteins of tumor cells.The structure, the physical and chemical character, and relation between structures and effect of the platosammine fatty acids has been researched by the method of X-ray diffraction, Forier transform infrared spectroscopy, differential thermal analysis and ultimate analysis. Ultimate analysis was showed the following: theoretical value tallies with experimental values. Experimental results of Forier transform infrared spectroscopy show the following: the functional group agrees with the structure. The result of X-ray diffraction was showed the following: The structure of the platosammine fatty acids is monoclinic system. In the experiment, human hematopoietic cell K562 is elected the model of ectoderm; human melenoma cell LiBr is elected the model of mesoderm; human cancer cell of the liver 7721 is elected the model of ectoderm. The platosammine fatty acids were determined by means of MTT test on the inhibiting effect of the growth of human tumor cells. The experimental results showed that the inhibiting effect of the growth has a direct bearing on the concentration and the length of hydrocarbon chain. The influence of magnetic field on the interaction between platosammine fatty acid and human tumor cells showed that the same cell line has different sensibilities to treated...