Investigation On Differential Expressed Genes In Bone Marrow Between Tibetan And Han Ethnic Group

Tibetan is unique ethnic group which exhibit best adaptation to the hypoxic environment at high altitude. Investigating differential expressed genes in bone marrow between Tibetan and Han ethnic group would be helpful to explore the mechanism of Tibetan adaptation to hypoxic environment and to develop the measurements in promoting hypoxia acclimatization of sea-level residents.In order to screen the specific genes to high altitude hypoxic adaptation , the SSH cDNA library of differential expression genes in bone marrow tissue between Tibetan and Han ethnic group was constructed and sequenced, and at mean time, the expression profile of differential expression genes between Tibetan and Han was analyzed by the gene chip method by using the bone marrow tissue samples from high altitude Tibetan natives and sea-level Han ethnic group. Some differential genes were reexamined by quantity real-time RT-PCR method.The reexamined result of real-time RT-PCR suggested that Hbε, Hbζ, Hbγ , BCL2, CCL5, IL28RA, IGFBPland TGFBR3 genes were upregulated , and TP53, BAX, EPOR, AQP1, Caspase2, Caspase8 和 CA2 genes were downregulated in the Tibetan bone marrow tissue compared with Han's.Compared with Han bone marrow myeloid tissue, genes encoded enzymes concerned with glycolysis, aerobic metabolism , genes encoded Complex I, IV, V of electron transport chain were downregulated in the Tibetan's, furthermore cell cycle-related genes, and RBC framework structure protein genes in Tibetan's were almost downregulated too. On the contrast, the hemoglobin genes, anti-apoptosis genes, some cytokine and chemokine genes are upregulated in the Tibetan's.These findings indicated that the effects of high altitude hypoxia on Tibetan bone marrow may be the result of multicultural genes' regulation. And the long-term...