Primary Study On The Structure And Function Of The Novel Mouse Gene Msid2

RNA interference (RNAi) has been shown to spread from cell to cell both in plants and Caenorhabditis elegans, this phenomenon was named systemic RNAi. The gene sid-1, which was found by Wiston et al in 2002, is responsible for the spreading of RNAi between cells. It is found that Caenorhabditis elegans will lose the RNAi spreading character if sid-1 is mutanted.Until now, sid-1 homologous genes have been identified in mouse, rat and human, but little is known about the function of these genes. In order to study the mechanism and function of these genes in mammal cells, we cloned the sid-1 homologous gene in mouse. Sequence analysis showed that the CDS of this gene we got has 15 bp longer than BC025888. So we think it may be a novel mouse gene .We named it msid2 and subjected it to GeneBank, the accession number is AY562214.Then we analyzed its gene structure, the chromosomal location. The Result showed that the msid2 gene is located on the mouse chromosome 16B4 .The genomic sequence of this gene is 16 kb in size. The Coding sequence of msid2, 1353bp in length, is composed of 14 exons, which encodes a protein of 450 amino acids. The bioinformatics analysis of mSID2 indicates that it possess two Leucine zipper motif (110-131aa, 117-138aa), a DED domain (82-145 aa) and a cyclin domain (374-409aa). Using RT-PCR, we found that msid2 was specially expressed in cerebrum and cerebellum. The in situ hybridization results revealed that msid2 was predominantly expressed in the central nervous system from embryonic day E10.5 to E17.5, and it is specifically expressed in neurons of contex, hippocampus and spinal cord.CHO and COS-7 cells were transiently transfected with pEGFP-N1 recombinant plasmids, and the subcellular localization of the expressed different fragments of mSID2...