Dissertation Identification Of Human Novel Genes And Protein Sequence Motifs

Present dissertation consists of three parts: the first part describes the silicon cloning and subsequent experimental pilot functional analysis of 154 human novel genes; the second part describes the in silico analysis of gene expression data of 17 human tissues, as well as the third part describes the identification of two novel protein sequence motifs.In the work described in the first part, mainly via ESTs strategy, combined with some other methods proposed by author, the author predicted and isolated 154 novel human cDNA genes, about 1/3 of which have been verified by subsequent PCR amplification in various human tissues. Experimental verification of other genes is going on. These genes cover a wide range of types according to their different functions, which are postulated to be involved in diverse life processions. The putative proteins of these genes were classified according to both GO and Panther methods. These genes are a valuable reservoir for further investigation and potential for related application. The identification and pilot functional analysis of human RAB41 and some other genes, e.g., RAB41, ASB-17, LPAAT-ζ s. well as GLYTK-1 and GLYCTK2 were introduced as examples, to elucidate various recruited cloning methods.In the second part, the author analyzed the gene expression data of 760 Unigenes among human 17 tissues via digital differential display approach. And the results indicated that human brain and testis exhibits a most similar gene expression pattern, which implies that both human brain and testis might play important roles in human speciation and evolution.In the third part, the identification of two novel protein sequence motifs MANSC and MPPN was introduced. MANSC is present exclusively in multiple cell animal membrane or extracellular proteins, including low density lipoprotein receptor-related protein 11 (LRP-11), Hepatocyte growth factor activator inhibitor 1 (HAI-1) and some other unknown membrane or extracellular proteins. We postulate that the MANSC domain in HAI-1 might function through binding with hepatocyte growth factor activator (HGFA) and matriptase. MPPN is highly related to the known domain rrm which serves to recognize and bind RNA molecules, thereby we predicted that MPPN carries out the similar roles to rrm with potent roles in cell cycle.