Application Of Microsatellite DNA Polymorphisms And DNA Fingerprints To Inbred Strain Mice And Rats

Application of microsatellite DNA Polymorphisms and DNA fingerprints to inbred strain miceand ratsTo screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite DNA Polymorphisms and DNA fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method.The study had established the foundation of the molecular genetic monitoring marker method of laboratory animal.Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically HRP labeled JL-02 by the second institute of the Public Safety Bureau of China and southern blot hybridization, the author studied many fingerprints of the same DNA, the different organic fingerprints of the same organism and fingerprints of parent and offspring. The patterns were completely different among the different strains and those of the samples from the same strain were completely identical. These bands of the offspring' fingerprint of were found in their parents, and other bands couldn't found. The fingerprint which had many fragments was sensitive and distinguishable, and had genetic and organic stability and high DNA polymorphism.Four strains of mice and rats including BALB/c, C57, 615, DBA/2 were analyzed by DNA fingerprint. 14 fragments were distinguishable and the length of these fragments was 2.0kb-23kb. The result showed that DNA fingerprint of the same strain was completely identical. The band-sharing rate in different individuals of the same inbred strain was 0.94-1.0 and the similarity coefficient in the same strain was 0.859~0.898. But The band-sharing rate in the different strains was 0.11 and the similarity coefficient was 0.11. The mother mouse and the offspring in the same kin of the two strains had the same fingerprints.DNA fingerprints of 13 colonies of 5 strains, including 5 BALB/c, 2 BALB/c-nu/nu> 4 C51 1 CBA/N and 2 DBA/2 from 7 factories of laboratory animal in the Beijing and Xi'an areas, were studied with JL-2 Mulilocus probe and southern hybridization and the author compared the DNA fingerprint with the biochemical marker enzyme method. It indicated that the fingerprint had 17-22 distinguishable fragments and these fragments had high polymorphisms. The fingerprints of BALB/c and BALB/c-nu/nu, which were abnormal at the Hbb site with the biochemical marker enzyme method, was different from their normal groups. The band-sharing rate and the similarity coefficient between BALB/c and BALB/c-nu-nu were below 0.8. the probability of the completely identical fingerprints between two strains was below 1.3x10"2. The strains which were normal with the biochemical markers analysis had the identical fingerprints and P was above 2.2x10-1. The fingerprints of the same strain of different laboratory animal factories was different and it's band-sharing rate and similarity coefficient were below 0.8 (P<1.1 x10-3), The band-sharing rate and similarity coefficient have great different in the different strains and it's probability was below 0.4 (P=2.7x10-10) . The band-sharing rate and the similarity coefficient in the BALB/c and BALB/c-nu/nu were below 0.65 (P=3.8x10-6). DNA fingerprint could analyze the genetic change accurately and found the genetic change which couldn't befound with the biochemical markers method. Comparison of the two methods showed that the DNA fingerprint was superior to biochemical marker, because it was sensitive, stable and it could not only distinguish strain from strain, strain from substrain, but also showed the variation level, kindred and distance between individuals, stocks and strains.9 colonies from 7 inbred rat strains were studied by DNA fingerprint. The result indicated that the DNA fingerprints had the great difference between the different strains, the average fragments were 16.360+2.178, the band-sharing rate was 0.061 +0.008., the .similarity coefficient was 0.062+0.008 and the rate having the same DNA fingerprint b...