Studies On Armillaira Biological Species In Heilongjiang Province Of China

Altogether 76 strains of Armillaria from several main forest regions in Heilongjiang Province were identified with mating test using tester strains of five known Armillaria biological species in Northeast China. These isolates belonged to the five species respectively, which included Armillaria sinapina, A.gallica, A.luteopileata, A.ostoyae and CBS F (A.singulal). The distribution, growth rhythm, hosts range and culture characteristics of the five Armillaria biological species in Heilongjiang Province, were also studied and discussed in the text. As Armllaria gallica was in the majority in the basidiocarp specimens, and occured frequently in Heilongjiang Province. It also was a cosmopolitan species in the Northern Hemisphere. The phylogenetics relationships between the isolates of A. gallica from Europe, North America and China were studied with ISSR-PCR amplification. The result indicated that the isolates of Armillaria gallica from the three continents belonged to their own phylogenesis lineages respectively. The genetic differentiation of these A. gallica strains for geographical isolation reason has almost formed completely. The genetic relationship between isolates of Europe and China was closer than China and North America's. The inner genetic diversity of Chinese phylogenesis lineages is higher, at the same time the territorial genetic differentiation and evolution direction divergence have taken place. The mixed lineage in Heilongjiang Province indicated that the isolates distribution pattern of A.gallica in Heilongjiang Province accorded with the characteristic that the distribution was formed by adaptive radiation of common ancestral forms.