Studies On The Signal Transduction Mechanism Of 50Hz Magnetic Field And The Inhibition Of Noise Magnetic Field

Electrification is the landmark in the civilization of mankind. However, evidence is accumulating that exposure to extremely low frequency electromagnetic field may produce a lot of biological effects. Especially, some reports showed that ELF-EMF such as those from electric power transmission and distribution lines have been associated with increased risk of childhood leukemia, cancer of the nervous system, and lymphomas. In order to find an explanation and to determine the possible mechanism of these biological effects, many in vitro and in vivo studies of EMF effects have been published. These studies were involved in many enzymes (such as ODC, succinate dehydrogenase, PKC), pro-oncogene (c-myc, c-fos, etc.), gap junction and protein synthesis, and so on. But the mechanism of the biological effects of ELF-EMF is still unclear.Low-frequency electromagnetic fields in our environment often have little energy to directly traverse the membrane, and act with biological molecules, or damage the DNA. It is possible that they may modify the existing signal transduction procession in cell membrane, thus producing both transduction and biochemical amplification of the effects of the field itself. So the related mechanism studies of the cellular signal transduction have advantages for investigating biological effects of ELE-EMF. The related studies showed that the stress-activated protein kinase (SAPK) and P38 mitogen-activated protein kinase (P38 MAPK) signal transduction pathways are universally existing in the mammalian cells, which transduct the environmentalphysical stresses into cell and produce biological effects through the cascade phosphorylation between proteins. ELF-EMF is also a physical factor. We suppose that these signal pathways may mediate 50Hz magnetic field (MF) signals in cells. The purposes of this study were to investigate the possible effects of exposure to 50Hz MF on protein tyrosine phosphorylation, SAPK signal pathway and P38 MAPK signal pathway. Cellular membrane is the possible action site for ELF-EMF, and the related studies confirmed that the receptors on cell membrane could be clustered by physical stress factor, and the receptors transduced the signal into cells via cellular signal pathway. Thus, we also investigated the effects of ELF-MF exposure on clustering of EGF, TNF receptors in cultured cells.Recently, Litovitz et al raised the spatial coherence theory of electromagnetic field (EMF). The theory thinks that regular MF is spatial coherence field and noise MF is spatial incoherence field, the noise MF could inhibit the biological effects induced by MF. Following it, there are serial experiments to support this theory. However, the inhibition effects of noise MF on biological effects induced by MF were not accepted universally because the intensity of MF which used in these experiments was too small (at micro-Tesla order) to produce the certain biological effects. So the theory is controversy. We think the inhibition effects of noise MF may be existence at theory level. In order to confirm the effects of noise MF, in the present study, we use 0.4mT MF and noise MF, which intensity is hundred times than the previous studies and is proved repeatedly in our experiments to produce the clear biological effects, to examine the effects of noise MF on the clustering of EGF, TNF receptors and SAPK phosphorylation induced by 50Hz MF.1. Effects of 50Hz Magnetic Field on Protein Tyrosine Phosphorylation in Cultured CellsProtein cascade phosphorylation is one of the main modes of signal transduction pathway and protein activation in cells. In order to investigate the effects of 50Hz MF exposure on protein tyrosine phosphorylation, Chinese Hamster Lung (CHL) cells were cultured routinely 3 days in MEM medium. After treated as follows: (1). Control, without any treated; (2). 0.4mT MF exposure, exposed to 0.4mT MF for differentdurations; (3). 0.8mT MF exposure, exposed to 0.8mT MF for different durations, the cells were harvested and protein was extracted. Western blotting...