Effects Of Different Intervention Time Points (扌衮) On Motor Function, Muscle Electrophysiology And Expression Of Inflammatory Factors In Rats After Skeletal Muscle Injur

Objective: To observe the effects of the rolling manipulation on the motor function(grip strength and balance),muscle electrophysiological electricity,and the expression of TGF-β1 and IL-6 inflammatory factors in serum and injured muscle tissues in vivo in rats with quadriceps injury at different intervention time points,and to explore the mechanisms related to the repair of quadriceps injury in rats by the rolling manipulation and the optimal time points for the intervention of the rolling manipulation on skeletal muscle injury.Methods: Eighty Waster rats of both sexes were randomly divided into five large groups: 2h,24 h,48h,72 h,and 96 h groups,with 16 rats in each group,and each group was further divided into 4 subgroups: 4 rats in the blank subgroup,4 rats in the model subgroup,4 rats in the treatment subgroup and 4 rats in the Yunnan Baiyao subgroup.All rats in the blank subgroup were injected with 400 ul of saline,and all rats in the model subgroup,treatment subgroup,and Yunnan Baiyao subgroup were injected with 450 ul of tiger snake toxin for molding,and after successful molding,the team’s homemade rolling massager treatment was administered at different time points: twice daily(at 10 am and 5 pm)for 3 minutes each time for 3 days;Yunnan Baiyao subgroup Aerosol administration treatment was carried out,with 2 sprays administered twice a day;the model and blank subgroups were reared normally.After each subgroup had completed 3 days of treatment,motor function tests were performed on the second day,followed by muscle electrophysiological tests,and finally,blood was taken from the abdominal aorta and from the most severely damaged area of the muscle(marked)to detect the expression of the inflammatory factors TGF-β1 and IL-6 in the tissues using ELISA.Results: grip strength test: the treated group in each of the five major groups had better grip strength than the model group(r<0.05),blank group > treated group > Yunnan Baiyao group >model group(r<0.05).Balance beam test: the balance of the treated group in each of the five major groups was better than that of the model group(P<0.05),model group > Yunnan Baiyao group > treatment group > blank group(r<0.05);muscle electrophysiology: in the test of electrophysiology,the mean amplitude of the quadriceps muscle in each group was compared,blank group > treatment group > Yunnan Baiyao group > model group(r<0.05).The serum and tissue levels of TGF-β1 and IL-6 in each of the five groups were lower in the treated group than in the model group,and the inflammatory response was significantly lower(r<0.05),and the serum and tissue levels of TGF-β1 and IL-6 in the 48 h group were model group > Yunnan Baiyao group > treated group > blank group(r<0.05)at different time points of the rolling manipulation of intervention.Conclusion: After quadriceps injury in rats,repair by rolling manipulation treatment was effective except for the 2h group;The rolling manipulation intervention starting at 48 h was more effective for balance repair,muscle electrophysiology,and inflammation regulation after quadriceps injury in rats;the rolling manipulation intervention starting at 96 h was the most effective for grip strength repair after quadriceps injury.