Study On The Correlation Between Acute Mastitis During Lactation And Milk Flora And Intestinal Flor

Objective: This paper mainly discusses the causes of mastitis and its relationship with intestinal bacteria,to further explore the pathogenesis of acute mastitis during lactation and to support clinical prevention and standardized treatment.Methords: Twenty breast milk samples and 20 stool samples were collected from July2020 to July 2021 from lactating women with acute mastitis and from normal lactating healthy women in our hospital.Samples were subjected to throughput sequencing of 16 S ribosomal RNA(r RNA)to analyze the correlation between the structure and the number of bacteria detected in the group of patients with acute mastitis during lactation and in the group of healthy women with normal lactation.Results: Twenty patients with acute mastitis during lactation and 20 healthy lactating women participated in this study,a total of 7801201 tags were obtained,with an average of97,515 tags per sample and a SD of 6672;the average tag length was 303 bp and the SD was6 bp.At 97% similarity,a total of 6215 OTUs were generated for 80 samples,with 2841,2271,3140,and 2933 OTUs for groups A,B,C,and D,respectively.The number of OTUs shared by A and B was 1914,1850 for C and D,908 for A and C,and 779 for B and D,respectively.Analysis based on cluster plots of species abundance,histograms of species profiles,alpha diversity,and blood values showed that species diversity in milk flora was statistically lower in lactating patients with acute mastitis than in healthy lactating women(P <0.01).PCA analysis based on OTU frequency showed significant differences in breast milk flora in lactating patients with acute mastitis compared to the species composition of healthy lactating women.Among the 40 breast milk samples,the abundance of Staphylococcus spp.among bacteria isolated from the case group ranked first,with S.aureus occupying 71.37% of the first place,followed by S.epidermidis 9.06%,while the abundance of Staphylococcus spp.among bacteria isolated from the control group was 40.71% and S.aureus 19.71%.The species frequency in the case group was higher than in the control group(P < 0.05),which was statistically significant.It belongs to several levels of biological species richness.The direct direction of hot soil and biological species walking shows that the richness of Lactobacillus is low.and Bifidobacterium spp.in the milk flora of the case group than in the control group,and Enterococcus spp.The substance appeared in the milk of the actual example group,but did not appear in the reference group.Among the 40 stool samples,the frequency of Staphylococcus spp.species in the case group was higher than in the control group at the genus level,and the frequency of S.aureus species was fifth,whereas the frequency of S.aureus species in the control group was extremely low,the two groups compared data appears to be very big difference between each other(P < 0.05).The frequency of Lactobacillus spp.The comparison between the disease instance group and the reference group was statistically significant(P < 0.05).Analysis of routine blood counts showed that white blood cell count,neutrophil count,and C-reactive protein were higher in the case group than in the control group(P < 0.05),this shows a very large statistical value.Conclusions: the occurrence of mastitis during lactation is related to the dysbiosis of the milk flora,the loss of regulation of milk bacterial community is associated with the loss of regulation of intestinal bacterial community,,and the inflammatory response of the body is involved in the occurrence of mastitis during lactation.