| Objective: By investigating effects of pressing manipulation on quadriceps tendon on cyclic adenosine monophosphate(c AMP),protein kinase A(PKA)and γ-aminobutyric acid(GABA)in spinal cord tissue of rats with ischemic poststroke myospasm(IPSS)and to explore mechanism of pressing to alleviate ischemic poststroke myospastic condition.Methods: Forty-five numbered rats were arranged by SPSS 26.0 to assign a random number size,6 rats in blank control group and sham-operated group respectively and the rest rats were used as model preparation group,rats that met successful modeling criteria were randomly assigned to permanent middle cerebral artery occlusion(p MCAO)model group and pressing manipulation treatment group,finally meeting 6 rats each group.All rats weighed 280-300 g before modeling,were fasted and water-freed before surgery.On the day of surgical operation(0d),operations other than ligation and embolization wire insertion were performed in sham-operated group,and model preparation group was performed by embolization of middle cerebral artery using modified ZeaLonga embolization wire method on right.Rats in preparation group were assessed for neurological impairment 1 day after surgery using Zea-Longa score,rats with scores of 2 and 3 were subsequently assessed for spasticity using modified Ashworth scale,rats with scale of 1,1+,and 2 were finally considered successfully modeled.Intervention was performed from 2d to 6d postoperatively.Blank control group,sham-operated group,and p MCAO model group were strapped down,while pressing manipulation group used a homemade "pressed stimulator" to press quadriceps tendon on left.c AMP,PKA and GABA in L1-L3 segments of rats’ spinal cord were determined by enzyme-linked immunosorbent assay(ELISA).Results:1.ZL score: 1 d postoperatively,3 with score of 1,20 with score of 2,7 with score of 3,and 0with a score of 0 & 4.2.MAS : 27 rats meeting ZL score,6 with scale of 0,12 with scale of 1,5 with scale of 1+,2with scale of 2,2 with scale of 3,and 0 with scale of 4.Preintervention: compared with blank control group,no significant difference on spasticity scale in sham-operated group(P>0.05),spasticity levels increased in both p MCAO group and treatment group(P<0.05);compared with shamoperated group,spasticity levels increased in both p MCAO group and treatment group;compared with p MCAO group,no significant difference in treatment group(P>0.05),suggesting successful modeling.After procedure,compared with p MCAO group,spasticity of rats in treating group decreased(P<0.05),suggesting Tuina pressing alleviating limb spasticity in IPSS rats.3.Changes on cyclic adenosine monophosphate(c AMP),protein kinase A(PKA)and γ-aminobutyric acid(GABA)in L1-L3 segments of spinal cord: compared with blank control group,no significant difference in sham-operated group(P>0.05),content in both p MCAO group and pressing manipulation group decreased(P<0.05);compared with sham-operated group,content in both p MCAO group and treatment group decreased(P<0.05);compared with model group,content of three testing indicators in pressing group increased(P<0.05);suggesting that pressing manipulation treating quadriceps tendon increasing stimulation of content of c AMP,PKA and GABA in spinal cord.Conclusion:1.Pressing-treated quadriceps tendon decreasing degree of limb spasticity in rats with ischemic poststroke myospasm.2.Mechanism of action of pressing-treated improving ischemic poststroke myospasm related to upregulation of c AMP,PKA and GABA in spinal cord of rats. |
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