Modulatory Effects Of Ketamine On Mortality ,Serum Nitric Oxide And Cyclic Adenosine Monophosphate And Cyclic Guanosine 3,5-monophosphate In Myocardial Tissues Of Septic Rats

Objective To investigate the effects of ketamine (KET) on mortality, serum nitric oxide (NO) and cyclic adenosine monophosphate (cAMP) and cyclic guanosine 3, 5-monophosphate(cGMP) in myocardial tissues of septic rats. Methods There were two phases in the experiment. 1? Research on mortality rates : Fifty Sprague-Dawley rats were divided into five experimental groups. A Cecal ligation-perforation (CLP) was used as a sepsis model. (1)CLP group; ?2?SHAM group; (3)KET I group (CLP+5%KET10mg/kg, im, bid); ㎏ET II group (CLP+5%KET50mg/kg, im,bid); (5)KETIII group (CLP+5%KET100mg/kg, im, bid) . The mortality rates were observed during five days in five groups. 2, Research on serum NO , myocardial cAMP and cGMP: The lowest mortality rate KET group , SHAM and CLP group were introduced into this phase of experiment. Two hundreds and thirty Sprague-Dawley rats were divided into three experimental groups. (1)CLP group (n=30); (2)SHAM group (n=100); (3)KET group (n=100) .The blood and myocardial tissue of rats in the three groups were collected for assaying serum NO , myocardial cAMP and cGMP. Results ㏕he mortality rate of CLP rats in KETII group was the lowest when 5% ketamine was given infused via muscle at 50mg/kg two times per day. ㏕he concentrations of serum NO was significantly increased(P<0. 01) in CLP and KET group comparied with SHAMgroup after operation, and the levels of serum NO in KET group were lower (P<0. 01)than that in CLP group. (3)The concentrations of myocardial cAMP in CLP and KET group decreased significantly(P<0. 01) while myocardial cGMP rose significantly(P<0. 01) comparied with SHAM group. Between KET and CLP group, the concentrations of myocardial cAMP in KET group were higher(P<0. 01) than that in CLP, and the levels of myocardial cGMP were lower(P<0. 01) than that in CLP group. Conclusions Ketamine can inhibit serum NO , myocardial cGMP and increase myocardial cAMP, thus decrease the mortality of septic rats.