| Objectives :The structure and composition of intestinal flora in patients with idiopathic pulmonary arterial hypertension were studied by metgenomic high-throughput sequencing technology,and the metabolites of intestinal flora trimethylamine oxide and short-chain fatty acid related flora were analyzed to explore the possible influence of intestinal flora on idiopathic pulmonary arterial hypertension.Methods: This study included 20 patients diagnosed with idiopathic pulmonary arterial hypertension at Yan’an Hospital affiliated with Kunming Medical University as the group with idiopathic pulmonary arterial hypertension(IPAH group,n=20);And 20 healthy control groups(Healthy group,n=20),collected the clinical data of the above subjects for statistical analysis,collected stool samples of the two groups,extracted sample DNA for metagenomics detection,and conducted bioinformatics analysis on the sequencing data to analyze the composition and functional characteristics of intestinal flora in patients with idiopathic pulmonary arterial hypertension.Results: 1.Quality control results: the value of Q20(the percentage of base number with the sequencing error rate less than 0.01 in the data)in each sample is greater than95%,and the contigs in each sample are greater than 500 bp.This shows that the instrument is stable and the data quality is good.2.Comparison of dominant flora between idiopathic pulmonary arterial hypertension group and healthy control group: The abundance of Firmicutes and Bacteroidota was the highest at the phylum level,which were 53.16% vs 57.08% and 23.45% vs22.10%,respectively.The abundance of Proteobacteria was 7.23% vs 3.79%,respectively.At class level,the abundance of Clostridia and Bacteroidia were the highest,which were 43.78% vs 46.51% and 23.31% vs 21.96%,respectively.The abundance of Gammaproteobacteria was 6.85% vs 3.31%,respectively.At the order level,the abundances of Eubacteriales and Bacteroidales were 43.71%vs46.49% and23.31%vs21.96%,respectively.The abundance of Enterobacterales was 6.29%vs3.04%respectively.At the family level,the abundance of Lachnospiraceae was the highest,14.72% vs 18.90%,and that of Bacteroidaceae was 14.99% vs 14.97%,respectively.The abundance of Oscillospiraceae was 11.47% vs 9.10%,respectively.At the genus level,Bacteroides had the highest abundance,9.27% vs 6.38%,Ruminococcus 5.81% vs 3.52% and Phocaeicola 3.51% vs 5.53%.3.Difference analysis of microflora between idiopathic pulmonary arterial hypertension group and healthy control group: In the idiopathic pulmonary arterial hypertension group,the abundance of bacteria that promote TMAO synthesis was increased,They are Bacteroides,Klebsiella,Prevotella and Escherichia coli,Bacteroidetes s＿＿Alistipes＿finegoldii,s＿＿Bacteroides＿salyersiae,s＿＿Bacteroides＿cellulosilyticus;The abundance of bacteria that promote short-chain fatty acid synthesis decreases,They are Firmicutes,Bifidobacterium,Coprococcus＿comes,Megamonas,s＿＿Megamonas＿rupellensis and Negativicu Class tes and order Selenomonadales.4.By comparing the non-redundant protein database,it was found that the abundance of the gene translated into non-specific serine/threonine protein kinase enzyme increased in the idiopathic pulmonary arterial hypertension group,which acts as the enzyme binding receptor and can bind to TMAO and promote the synthesis of TMAO.The gene abundance of propionate Co A-transferase enzyme,choloylglycine hydrolase enzyme,betaine reductase enzyme and amidase enzyme(which can promote short-chain fatty acid synthesis)is decreased.Conclusions:1.Differences in gut microbiota structure between patients with idiopathic pulmonary arterial hypertension and the control group.2.In patients with idiopathic pulmonary arterial hypertension,the abundance of bacteria promoting TMAO synthesis increased,while that promoting short-chain fatty acid synthesis decreased.3.The imbalance of intestinal flora may play an important role in the occurrence and development of idiopathic pulmonary arterial hypertension. |
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