Correlation Of FGB Promoter Polymorphisms With Pulmonary Embolism

Objective :The presence of single nucleotide polymorphism in the beta chain within the fibrinogen promoter region was investigated.This was used as a basis for constructing a logistic regression model to reveal the effect of SNPs loci in the FGB promoter region on susceptibility to pulmonary embolism,haemodynamics and coagulation function in a genetic context.Methods: A total of 58 patients admitted to the Department of Vascular Surgery of the First Affiliated Hospital of Kunming Medical University from December 2020 to December 2022 and diagnosed with pulmonary embolism by CT pulmonary angiography were selected for this study.In the control group,a total of 114 patients were selected from the same time period of health screening.The subjects in both groups were between 18 and 80 years of age.Routine blood count,blood biochemistry,coagulation parameters and cardiac ultrasound parameters were collected from both groups.At the same time,about 5m L of whole blood samples were collected from both groups for sequencing of the FGB promoter region and recording their genotypes.Finally,the Hardy-weinberg genetic equilibrium test for SNPs loci was performed using relevant statistical software,and the differences in genotype frequencies of SNPs between the case and control groups,and the effects of the FGB promoter region on coagulation function and haemodynamics in patients with pulmonary embolism were analysed.Results: 1、statistically significant between the case and control groups in terms of gender,and not statistically significant in terms of age.2、statistically significant in the case and control groups in terms of white blood cell count,neutrophil count,monocyte count,red blood cell count and alanineaminotransferase.3、the genotype frequencies of individual SNPs were not statistically significant in both the case and control groups.4、β-993C/T genotype CC versus genotype CT+TT in the control group had statistically significant prothrombin time,with genotype CC having less prothrombin time than genotype CT+TT.β-455G/A genotype GG versus genotype AG+AA in the control group had statistically significant partial activation prothrombin time,with genotype GG having less partial activation prothrombin time than genotype AG+AA.β-455G/A was statistically significant in the right ventricular internal diameter and β-249C/T was statistically significant in the right atrial internal diameter in the experimental and control FGB promoter regions.5、In the case group β-1420G/A genotype GG and genotype AG+AA were statistically significant in FDP,and genotype GG had a smaller FDP than genotype AG+AA.β-854G/A genotype GG and genotype AG+AA were statistically significant in FDP in the case group,and genotype GG had a smaller FDP than genotype AG+AA.β-854G/A in the control group Genotype GG versus genotype AG+AA in the control group was statistically significant in FDP,with genotype GG having a greater FDP than genotype AG+AA.6、Hardy-Weinberg genetic equilibrium was achieved for all SNPs in the FGB promoter region in the experimental and case groups.7、In the case group and control group β-455G/A,genotype GG and AG+AA were statistically significant in terms of right ventricular internal diameter,with genotype GG having a smaller right ventricular internal diameter than genotype AG+AA.In the case group and control group β-249C/T,genotype CC and CT+TT were statistically significant in terms of right atrial internal diameter,with genotype CC having a smaller right atrial internal diameter than genotype CT+TT.Conclusion: 1 、 The study is well balanced in terms of age and genetics(Hardy-weinberg genetic balance)and is suitable for genetic studies.2、Men may be more likely to develop pulmonary embolism than women.3、No statistically significant differences were found in the genotypes of theindividual SNPs between the case and control groups.4、A gene in β-1420G/A,A gene in β-854G/A,T gene in β-993 C/T and A gene inβ-455G/A were found to be possible protective factors for pulmonary embolism in SNPs loci and coagulation function;no statistically significant differences were found in the remaining loci and coagulation function.5、A statistical relationship between β-455G/A and β-249C/T and the right atrium and right ventricle was found in the study of SNPs in the FGB promoter region and cardiac haemodynamics,with no similar findings at SNPs at other loci.6、In the natural course of pulmonary embolism,the thrombotic condition is subject to constant recurrence,and the blood may alternate between hypercoagulable and hypocoagulable states,presenting a dynamic balance that is sometimes difficult to define.