Relationship Between FGB Promoter Gene Polymorphism And Thrombotic Superficial Phlebitis

Objective:By exploring fibrinogen in patients with superficial thrombophlebitis(STP)β(FGB)promoter region,known or unknown single nucleotide polymorphisms(SNPs),to explore the genetic high-risk factors of STP,and to study the impact of SNPs in FGB promoter region on the coagulation function,incidence rate and other related factors of STP.Methods:1.This research focuses on exploring the possible genetic high-risk factors of SNPs in the FGB promoter region in patients with thrombotic superficial phlebitis.In order to reduce interference from other factors,especially in genetic aspects,both the case group and the control group in this study are Han people in Yunnan Province.Collect basic information and blood cell analysis,blood biochemical indicators,and coagulation function indicators(prothrombin time,fibrinogen,thrombin time,and activated partial thromboplastin time,etc.)for the above population.At the same time,the blood samples of the two groups of subjects were injected into a 1.5 ml EP tube mixed with heparin,and stored in-80℃liquid nitrogen for standby.2.The study of thrombophlebitis and polymorphism of FGB promoter region adopts the method of long segment gene sequencing to explore the whole promoter region of FGB gene,possible SNPs sites,and whether they are related to the incidence rate of STP,as well as the impact on blood cells,blood biochemistry and coagulation function(prothrombin time,fibrinogen,thrombin time and activated partial thromboplastin time).The main methods are:(1)Hardy-Weinberg genetic balance test of SNPs in FGB promoter region in case group and experimental group;(2)The genotypic frequency difference of SNPs in FGB promoter region between case group and control group was analyzed;(3)Mann-Whitney rank sum test and binary logistic regression analysis were performed on the blood cells,blood biochemistry and coagulation function of the case group and the control group;(4)Mann-Whitney rank sum test was used to analyze the effect of different genotype SNPs in FGB promoter region on blood coagulation function;Results:The research results show that:1.The SNPs gene in FGB promoter region of the case group and the control group were tested by Hardy-Weinberg genetic balance test,and it was found that between the two groups,exceptβ-248CT in the case group did not conform to the law of genetic balance(P<0.05),the other groups all conformed to the law of genetic balance(P>0.05);2.There was no significant difference between the case group and the control group in gender and age constituent ratio(P>0.05);3.After analyzing the blood cells,blood biochemistry and coagulation function of the case group and the control group,it was found that the monocyte count of blood cells was P<0.05,the fibrinogen degradation product in the coagulation function was P<0.05,and D-dimer=P<0.001(all P<0.05),with statistically significant differences.There was no significant statistical difference between the other indicators(P>0.05).4.Comparing the SNPs genotype frequency of FGB promoter region between the case group and the control group,we found that:β-The frequency of 455G/A genotype was statistically different between the case group and the control group(P<0.05),and the analysis of variance wasX~2=5.381(P=0.020);The binary logistic regression analysis showed that B=-1.268,P=0.025,95%CI=0.093～0.0852, suggesting that this gene locus may be a risk factor for STP.5.There was no statistically significant difference between different genotypes of SNPs in FGB promoter region on coagulation function and Fg synthesis.Conclusion(s):1.This study on SNPs in the FGB promoter region of STP has good pertinence in the region and population of the study object,and the factors such as sex,age and genetics of the study population are well balanced,which is suitable for genetic research.2.In the process of the onset,development and prognosis of SPT,due to the hypercoagulant function of the body leading to thrombosis,in order to maintain the balance and enhance the fibrinolytic function,the body will cause changes in the values of fibrinolytic indicators such as FDP and DD2;3.The occurrence of STP is accompanied by the occurrence of inflammation.If the inflammation reaction is mild,the whole blood cell changes are not obvious,and there may be only changes in the indicators of inflammation reaction in MONO;4.In 6 SNPs of FGB promoter region,β-455G/A gene may be a risk factor for STP...