| To assess Non-motor symptoms in Parkinson’s disease using cerebral blood flow reserve,exosome expression levels in peripheral blood.Methods Total of 42 Parkinson patients were selected to be screened from the outpatient and inpatient clinics during the period of Neurology in our hospital,and 40 age and sex matched healthy individuals were enrolled as the study control group during the same period.Inclusion criteria for the Parkinson’s disease group:(1)Referring to the Guidelines for Primary Diagnosis and Treatment of Parkinson’s Disease and the Diagnostic Standards for Parkinson’s Disease in China,two senior physicians diagnosed Parkinson’s disease as clinically confirmed;(2)The onset age is between 40 and 80 years old.Inclusion criteria for the control group: During the same period,40 healthy individuals matching the age and gender of the patient group were included as the study control group.Exclusioncriteria:(1)Patients diagnosed with Parkinson’s syndrome and Parkinson’s syndrome,and other neurodegenerative diseases.(2)Tumors,severe craniocerebral trauma,peripheral and central system inflammation,infection,or autoimmune diseases Those who have severe dysfunction of major organs such as the heart,liver,lungs,and kidneys,as well as significant cognitive impairment,and are unable to cooperate with the examination;(4)Lower limb venous thrombosis and lower limb arterial stenosis or terminal arterial occlusion;Patients with recent myocardial infarction or cardiac arrhythmia.(5)All selected cases signed an informed consent form for research ethics.Cerebral blood flow velocity was monitored in all patients with a custom made cerebral blood flow reserve monitoring head frame.And arterial blood pressure was collected synchronously by non-invasive fingertip blood pressure dynamic continuous monitoring.All cerebral blood flow velocity CBFV,arterial blood pressure ABP data were processed and analyzed by the function transfer method using cvr200 application software.All the people was collected 10 ml of peripheral venous blood in the early morning and subjects was collected for exosome detection.All the people were evaluated with non motor symptom rating scale(NMSS)of Parkinson’s disease.The measurement data were expressed as mean(m)± standard deviation(SD),and the counting data were expressed as rate.The measurement data were analyzed by t-test,ANOVA,and linear regression analysis for correlation.Results NMSS analysis,control group 38.28 ± 32.26;test group 88.26± 56.07,was statistically significant,analysis,t = 4.916(P < 0.05).For the phase difference statistic,there was no statistical difference between the left and right sides of the test group and the control group,so the post data analysis was performed based on their mean values,test group111.25 ± 26.25;Versus 147.09 ± 27.51 in the control group,a statistical t-test was performed and a t= 6.038(P < 0.05)was considered statistically significant.The level of plasma exosomalmir-331-5p in test patients was(0.86 ± 0.43),which was significantly higher than that(0.21 ± 0.90)in the control group(t = 9.342,P < 0.005).There was a linear regression relationship between cerebral blood flow reserve and NMSS scores,and between exosome expression levels and NMSS scores.Conclusions:1Cerebral blood flow reserve is significantly decreased in patients with Parkinson’s disease.2.Patients with Parkinson’s disease have lower cerebrovascular flow reserve and more severe non motor symptoms.3.Higher exosomal mir-331-5p expression in PD patients was more severe non motor symptoms. |
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