Protective Effects Of Cannabidiol On Renal Ischemia Reperfusion Injury In Mice

Objective:To investigate the protective effect of Cannabidiol（CBD）on ischemia reperfusion injury in mice kidney.Methods:32 8-week-old WTC57BL/6 male mice were randomly divided into 4 groups（n=8 each）:They were divided into sham operation group（sham group）,renal ischemia reperfusion group（IR group）,low dose cannabidiol group(5mg·kg^-1,IR+CBD+L group)and high dose cannabidiol group(10mg·kg^-1,IR+CBD+H group).The mice were anesthetized according to their body weight.After successful anesthesia,only bilateral renal pedicles were dissociated in sham group.In IR group,IR+CBD+L group and IR+CBD+H group,the bilateral renal pedicles were clipped with arterial clips and the renal blood flow was blocked for 35min.The mice in sham and IR groups were intraperitoneally injected with placebo(0.025ml·g^-1,1%Tween-80+normal saline),and the mice in IR+CBD+L group were intraperitoneally injected with CBD(0.2mg·m L^-1,1%Tween-80+normal saline)at a dose of 5mg·kg^-1immediately after the removal of the clips that clipped the bilateral renal pedicle.In IR+CBD+H group,CBD(10mg·kg^-1,0.4mg·ml^-1,1%Tween-80+normal saline)was intraperitoneally injected immediately after removal of the bilateral renal pedicle arterial clips.Venous Blood and bilateral kidneys of mice were obtained 24 hours after restoration of renal blood perfusion.Serum Creatinine（Scr）and Blood Urea Nitrogen（BUN）were measured.BUN),Tumor Necrosis Factor Alpha（TNF-α）and Interleukin-1β（IL-1β）levels;The contents of Superoxide Dismutase（SOD）and Malondialdehyde（MDA）in renal tissue were detected.The pathological changes of renal tissue were observed under light microscope.Results:Compared with sham group,the levels of Scr,BUN,IL-1β,TNF-α,MDA and renal pathological injury score in IR group were significantly higher（P<0.05）,while SOD level was significantly lower（P<0.05）.Compared with IR group,the levels of Scr,BUN,IL-1β,TNF-ιand MDA in IR+CBD+L group and IR+CBD+H group decreased significantly（P<0.05）,and the SOD level increased（P<0.05）.Conclusion:CBD can protect the kidney from ischemia-reperfusion injury in mice,and its protective effect may be related to reducing inflammatory responses and inhibiting oxidative stress.