Study On The Expression Level Of Inflammatory Factors In Blood And Tissue Of Primary Varicose Veins Of Lower Extremities

Background: The pathogenesis of primary varicose veins in the lower extremities has not been fully elucidated.Currently,many studys have focused on genetic mutations,inflammatory mechanisms,oxidative stress,and the impact of sexual hormone levels.Reviewing of research progress at home and abroad,one of the most controversial issues in the pathogenesis of primary varicose veins of the lower extremities is whether inflammation exists in them,and how inflammation promotes the occurrence and development of varicose veins of the lower extremities.Objective: This study aims to determine which inflammatory factors are differentially expressed in the blood and tissues of patients with primary varicose veins of the lower extremities,and explores the inflammatory mechanism and the role of NF-κB inflammatory pathway in the occurrence and development of primary varicose vein,and compares and analyzes the changes of inflammatory factor levels in the blood of varicose veins of the lower extremities after 1 week of treatment with mucopolysaccharide polysulfonate cream.Methods: In this study,we collected the blood from 55 patients with primary varicose veins of the lower extremities(CEAP grade C2-C4)at the site of calf varicosity,and the blood from 55 normal individuals at the site of calf varicosity.The serum was obtained by centrifugation,and the expression of 20 inflammatory factors was detected using a high throughput liquid phase protein chip method.At the same time,we collected the varicose vein tissue removed during primary varicectomy of the lower extremities(3 cases)and the remaining normal great saphenous vein tissue during coronary artery bypass grafting(3 cases),and performed Western blot to determine NF-κB p65 expression level.In addition,55 patients with primary varicose veins of the lower extremities were treated with local application of mucopolysaccharide polysulfonate cream for one week,and blood was drawn from the same varicose site again.The expression levels of IL-1β,IL-17 A and IP-10 were measured by highthroughput liquid phase protein chip method.Results: The expression of IL-1β,IL-17 A and IP-10 in the blood of patients with varicose veins in the primary lower extremity varicose vein group was higher than that in the normal group,and the difference was statistically significant(P<0.05);IL-1β was expressed with the aggravation of symptoms(higher CEAP grade C grade),and the expression was also higher(r=0.339,P=0.011).The expression of NF-κB p65 in varicose vein tissue was higher than that in normal venous tissue,and the difference was statistically significant(P<0.05),and the expression level of IL-1β was significantly reduced after 1 week of treatment with mucopolysaccharide polysulfonate cream in primary varicose veins of the lower extremity(P<0.01).Conclusion: The expression of inflammatory factors IL-1β,IL-17 A and IP-10 in patients with primary varicose veins of the lower extremities was higher than that of normal people,suggesting that inflammation existed in varicose veins.IL-1β was related to the progression of primary varicose veins of the lower extremities,and at the same time,after treatment with mucopolysaccharide polysulfonate cream,the level of IL-1β was significantly reduced,indicating that IL-1β was an important inflammatory indicator for the occurrence and development of primary varicose veins of the lower extremities.NF-κB p65 overexpression suggests that NF-κB inflammatory pathway may be involved in the occurrence and development of primary lower extremity varicose veins,which needs to be verified by further experiments.