| ObjectiveIn this study,the changes of inflammatory factors and T lymphocyte subsets produced by humoral immunity in patients with ascites of viral hepatitis cirrhosis were detected,and the correlation between these indicators and ascites of viral hepatitis cirrhosis was investigated,aiming to provide reference for clinical prevention and treatment of this disease.MethodsFrom January 2019 to June 2021,100 viral hepatitis cirrhosis ascites patients were selected as the study group,and 100 viral hepatitis patients were matched as the control group.Inclusion criteria:Research group:(1)Consistent with the diagnostic criteria of viral hepatitis cirrhosis ascites disease.(2)The age of the patients are over 18 years old,regardless of gender.(3)Be aware of the study and agree to sign an informed consent.Control group:(1)Consistent with the diagnostic criteria of viral hepatitis.(2)Match the patients in the control group with the case principle of 1:1.(3)The age of the patients are over 18 years old,regardless of gender.(4)Be aware of the study and agree to sign an informed consent.(5)The selected cases were admitted to the hospital at the same time as the patients in the study group and were hospitalized in the same hospital.Exclusion criteria:(1)Patients with primary liver cancer.(2)Infected persons at other locations resulting from combined infection with Cytomegalovirus or vaccines.(3)Combine hematopoietic and endocrine system,urinary system,respiratory system,severe cardiovascular and cerebrovascular related diseases and non-viral hepatitis and non-viral hepatitis cirrhosis ascites.(4)Those who have intellectual and mental disorders and are unable to communicate.(5)Those who have undergone transhepatic hepatic shunt or splenectomy.(6)Patients with severe immunodeficiency diseases.All clinical data of patients were collected,including sex,age,occupation,ethnicity,family history of liver disease,knowledge level of viral hepatitis,pathogen classification,liver protection treatment,antiviral treatment,smoking history,drinking history,physical exercise,labor status,sleep status,etc.In addition,the peripheral blood of two groups of patients were taken at the time of admission,and the reagents provided by BD Company of USA were used to prepare relevant standard products and 6 types of cytokines microspheres(interleukin-2,interleukin-4,tumor necrosis factor-α,interleukin-6,gamma-interferon and interleukin-10)according to the instructions on the reagent kit,and 50μL of the serum to be tested were placed in the mixed capture microspheres and 50μL of PE dye,incubated in a room temperature environment for 3 hours without light,washed and suspended centrifugally,and then measured by a flow cytometer.In the process of measurement,the procedures are strictly operated according to the instructions on the reagent kit.Detection of T lymphocyte subsets:2 ml of peripheral venous blood of two groups of patients was extracted with reagent provided by BD company of USA,and was used for coagulation.One sample tube was taken as a flow type sample tube,and 20μL of CD3~+/CD4~+/CD8~+combined labeled antibody was added into the whole blood.After vortexing,the whole blood was incubated for 15 minutes in a room temperature environment without light,and then 450μL of hemolysin was added to incubate for 15 minutes in a dark light.After the patients were completely hemolysed,the samples were washed once more.After the samples were left standing and resuspended,the data obtained were analyzed by BD Cell Quest.Enter the collected clinical data into software Epidata 3.1,adopt double person audit recording to ensure data integrity and accuracy,use SPSS24.0 statistical software for data analysis,according to whether the data is normal or not,the counting data is expressed as[case(%)],andχ2 test is performed;Measurement data in(X±s),line t test;Logistic regression model was used to analyze the factors affecting the development of ascites in patients with viral hepatitis.P<0.05 was statistically significant for the difference.hepatitis E combined with hepatitis B ResultsAmong the 100 patients with viral hepatitis cirrhosis ascites in the study group,there were 79 cases(79.00%)of chronic viral hepatitis B cirrhosis ascites,15 cases(15.00%)of chronic viral hepatitis C cirrhosis ascites,2 cases(2.00%)of viral hepatitis A combined with viral hepatitis B cirrhosis ascites,4 cases(4.00%)of viral hepatitis E combined with viral hepatitis B cirrhosis ascites;63 cases(63.00%)of male patients,37(37.00%)cases of female patients.Among the 100 patients with viral hepatitis in the control group.There were 76 cases(76.00%)of chronic viral hepatitis B,17 cases(17.00%)of chronic viral hepatitis C,3 cases(3.00%)of viral hepatitis A combined with viral hepatitis B.4 cases(4.00%)of viral hepatitis E combined with viral hepatitis B;62 cases(62.00%)of male patients,38 cases of female patients(38.00%).There was no difference in gender and type of viral hepatitis between the two groups(P>0.05).The patients in the control group and the study group Comparison of demographic characteristics,age,sex,occupation,ethnicity,family history of liver disease,level of awareness of viral hepatitis knowledge,etiological classification,long-term antiviral treatment,heavy drinking,heavy smoking,physical exercise,There was no significant difference in insomnia,labor and other conditions(all P>0.05),which were comparable.Univariate analysis showed that the long-term antiviral treatment,interleukin-2(IL-2),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),interferon-There were statistically significant differences inγ(IFN-γ),CD3~+CD4~+,and CD4~+/CD8~+(all P<0.05).Logistic regression model analysis showed that the increase of INF-γin blood was positively correlated with the occurrence of viral hepatitis cirrhosis ascites(P<0.05).The increase of the cytokine tumor necrosis factor-α(TNF-α)in the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites(P<0.05).The increase of interleukin-2(IL-2)in the blood of patients was negatively correlated with the occurrence of viral hepatitis cirrhosis ascites(P<0.05).The increase of cytokine IL-6 in the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites(P<0.05).The increase of T-inducing/T helper cells(CD3~+CD4~+T cells)in the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites(P<0.05).The increase of CD4~+/CD8~+in the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites(P<0.05).Long-term antiviral treatment was negatively correlated with the occurrence of viral hepatitis cirrhosis ascites(P<0.05).Conclusion1.The increase of INF-γin the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites.2.The increase of tumor necrosis factor-α(TNF-α)in the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites.3.The increase of IL-2 in the blood of patients was negatively correlated with the occurrence of viral hepatitis cirrhosis ascites.4.The increase of cytokine IL-6 in the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites.5.The increase of T-inducing/T helper cells(CD3~+CD4~+T cells)in the blood of patients was positively correlated with the occurrence of viral hepatitis cirrhosis ascites.6.The increase of CD4~+/CD8~+in the patient’s blood was positively correlated with the occurrence of viral hepatitis cirrhosis ascites.7.Long-term antiviral treatment was negatively correlated with the occurrence of viral hepatitis cirrhosis ascites. |
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