Research Of Fecal Non-Targeted Metabolic Profile In Reproductive Age Women With Chronic Constipation

Objective:In our previous research,we found that there was a dysbiosis of gut microbiota in women of childbearing age with chronic constipation.However,there is no consensus on how their gut microbiota metabolic change.In this study,we plan to evaluate the clinical symptoms of chronic constipation patients in women of childbearing age on a scale,analyze the characteristics of differential metabolites of constipated patients’ fecal microbiota by non-targeted metabolomics technology,and explore the metabolic pathways that these metabolites may be participated in the progression of chronic constipation.Methods:(1)According to the Rome IV diagnostic criteria for functional constipation,women of childbearing age with chronic constipation(n=30)and healthy controls(n=30)were recruited as constipated groups and control groups,respectively,and eligible stool samples were retained.(2)Constipation symptoms,severity and quality of life of slow-transit constipated patients were assessed using scale,scoring system,and quality of life scales.And then classify the type of constipation.(3)Non-targeted metabolomics techniques were applied to analyze the differences in fecal metabolites between the case and control groups.Results:(1)According to the Bristol Stool Form Scale and Wexner Constipation Scoring System,women of childbearing age with chronic constipation mainly have type 1 and type2 stools,which are mostly colonic slow transit constipation.(2)Compared with the control group,the total PAC-QOL score and the scores of 4dimensions of somatic discomfort,psychosocial discomfort,worry and anxiety,and bowel satisfaction were significantly higher in the case group(P < 0.05).(3)Based on partial(OPLS-DA),significant differences were found in the metabolic profiles of fecal samples from constipated patients and healthy controls.Tryptophan,L-histidine,gamma-L-glutamyl-L-glutamic acid,and L-glutamine levels were significantly lower(p < 0.05)and tryptophan-derived 3-hydroxy-aminobenzoic acid levels were significantly higher(p < 0.05)in fecal samples from constipated patients.Differential analysis of the KEGG metabolic pathway indicated that tryptophan metabolism was significantly enriched in constipated patients.Conclusion:(1)Women of childbearing age with chronic constipation tend to have slow stool production,which has a negative impact on the patient’s life and significantly reduces the patient’s quality of life.(2)The metabolic profile of stool in women of childbearing age with chronic constipation differs significantly from that of healthy controls,with tryptophan being the metabolic pathway most affected.