Research Of Serum Non-target Metabolomics In Chronic Constipated Women Of Reproductive Age

Objective:Chronic constipation is a common clinical disease with a progressively younger population.Studies have shown the presence of intestinal microbiota disorders in chronic constipated women of reproductive age and the involvement of microbita metabolites in the development of disease.However,changes in the serum metabolites of patients with chronic constipation have not been fully clarified.Therefore,in this study,we used questionnaires and scales for symptom assessment,and non-target metabolomics to observe serum metabolic profiles in women with chronic constipation in their reproductive years.And we hope to provide a theoretical basis for the screening of serum metabolic targets in chronic constipated women of reproductive age.Methods:(1)Women of reproductive age with chronic constipation(n=30)and healthy controls(n=30)were recruited as constipated group and control group,respectively,and then evaluated in detail and qualified blood specimens were retained.(2)Constipation symptoms and quality of life of slow-transit constipated patients were assessed using questionnaires,constipation symptom and quality of life scales.(3)Observe the clinical metabolic index changes in patients by clinical indicators,such as body mass index,fasting glucose,liver metabolism and lipid metabolism indicators.(4)Analyze the altered serum metabolites of patients with chronic constipation in reproductive age by non-target metabolomics techniques.Results:(1)Chronic constipated women of reproductive age mainly had symptoms of slow-transmission colonic constipation.The total PAC-QOL score and the scores of 4assessment perspectives were higher in the constipation group(P<0.05).(2)The clinical indexes related to BMI,fasting glucose,liver metabolism and lipid metabolism were not significantly different between two groups(P>0.05).(3)The results of OPLS-DA analysis showed a significant difference in serum metabolites between constipated women of reproductive age and healthy controls.The levels of phenylalanine,L-leucyl-L-proline,N-formylmethionine,L-valine,and glycine in amino acid metabolism were significantly lower in the constipation group(P<0.05).And 2-hydroxyphenylacetic acid,as the derivative of phenylalanine,was up-regulated in the constipation patients(P<0.05).In addition,the levels of 3β-hydroxy-5-cholenoic acid were elevated in constipated patients(P<0.05).Pathway analysis indicated that phenylalanine metabolism,caffeine metabolism and glycine,serine and threonine metabolic pathways were most affected in chronic constipated women of reproductive age.Conclusion:(1)Chronic constipation in women of childbearing age is mainly characterized by symptoms of slow-transmission colonic constipation and reduced quality of life.(2)Serum metabolites were significantly different between constipated women of reproductive age and healthy controls.The pathways that were significantly affected were phenylalanine metabolism,caffeine metabolism and glycine,serine and threonine metabolism.