| Objective:To investigate the expression and the clinical significance of gelsolin(GSN)in cervical squamous cell carcinoma.Methods:We used the immunohistochemical staining method to detect the positive expression rate of GSN in 69 cases of cervical squamous cell carcinoma tissues,20 cases of cervical squamous intraepithelial lesion tissues and 20 cases of normal cervical tissues,and then we analyzed the correlation between the positive expression rate of GSN afnd the clinicopathological characteristics(including patients’ age,tumor diameter,lymph node metastasis,vascular invasion,degree of differentiation,and depth of invasion)of cervical squamous cell carcinoma patients.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of GSN in serum of 22 patients with cervical squamous cell carcinoma(preoperative clinical FIGO 2018 stage IA1-IIA)and 26 normal control patients.The differences in GSN expression levels were analyzed,and the correlation between GSN and the expression of squamous cell carcinoma antigen(Scc),a classical tumor marker of cervical squamous cell carcinoma,was also analyzed.And the prediction efficiency of GSN in cervical squamous cell carcinoma was further evaluated.Results:The positive expression rate of GSN in cervical squamous cell carcinoma tissues(78.26%)was higher than that in cervical squamous intraepithelial lesion tissues(40.00%)and normal cervical tissues(45.00%),and the differences were statistically significant.The positive expression rate of GSN in cervical squamous intraepithelial lesions tissues(40.00%)was not statistically significant compared with that in normal cervical tissues(45.00%).The positive expression rate of GSN in cervical squamous cell carcinoma was related to the degree of tumor differentiation and depth of invasion.The positive expression rate of GSN in low-differentiated cervical squamous cell carcinoma(90.00%)was higher than that in medium-well-differentiated carcinoma(69.23%).The positive expression rate of GSN in cervical squamous cell carcinoma tissues with infiltration depth ≧1/2 muscle layer(90.32%)was higher than that in infiltration depth <1/2 muscle layer(68.42%).And the differences were statistically significant.There was no significant difference in the positive expression rate of GSN in cervical squamous cell carcinoma with different ages,tumor diameter,lymph node metastasis or vascular invasion.The serum GSN expression level in patients with cervical squamous cell carcinoma is(17.94±8.20)ng/ml,which was higher than that in healthy control group(5.41±3.17)ng/ml,and the difference was statistically significant.The expression level of GSN was positively correlated with that of Scc,and the correlation coefficient was 0.599.The area under ROC curve of Scc and GSN in the differential prediction of cervical squamous cell carcinoma was 0.861 and 0.948,respectively.The area under ROC curve of Scc and GSN combined in the prediction of cervical squamous cell carcinoma was 0.967.The prediction efficiency of combined detection is higher than that of each index alone.Conclusion:GSN is highly expressed in both cervical squamous cell carcinoma tissue and serum,suggesting that GSN may play an significant part in promoting the development of cervical squamous cell carcinoma.The positive expression rate of GSN increased with the decrease of tumor progression and differentiation,suggesting that GSN may be a potential marker for predicting the prognosis of cervical squamous cell carcinoma.There was a significant positive correlation between GSN and Scc expression in serum.The combined detection of GSN and Scc has important predictive value for cervical squamous cell carcinoma. |
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