Effects Of Naringenin On Bone Metabolism In Patients With Type Ⅱ Diabetes Mellitus

Objective:To investigate the effect of naringenin on bone metabolism in type 2 diabetic mice.Methods:Based on the successful establishment of T2 DM model in mice,naringenin(75mg/kg and 300mg/kg)was administered orally for 4 weeks.After the experiment,the mice were sacrificed and serum TRAP,ALP,BGP and D-LA levels were measured.The left femur of the mice was harvested and stained with HE and TRAP.Results:After naringin treatment,the blood glucose of mice showed a step-like decline trend within 4 weeks,and there was a statistically significant difference between the initial blood glucose value and the blood glucose value after gavage(P < 0.01).Compared with the control group,the serum TRAP,BGP,ALP and D-LA were significantly reduced.There was no statistical difference between the 75mg/kg group and the control group,but there was a statistically significant difference between the 300mg/kg group and the control group(P < 0.01).There were statistically significant differences between BGP and D-LA in 75mg/kg group and 300mg/kg group(P < 0.05).The levels of Tb.Ar,Tb.Pm and %Tb.Ar in the two experimental groups were significantly increased and had a dose effect,but compared with the model group,the levels of Tb.Th and Tb.N were increased but not obvious,while the levels of Tb.Sp were significantly decreased.There was no significant difference between the two concentration groups.Conclusion:Our results demonstrate several important points related to bone metabolism and naringenin in T2 DM mice.Firstly,T2 DM can lead to osteoporosis.Secondly,naringenin delayed bone loss induced by T2 DM,improved femoral microstructure and regulated serum bone metabolism related parameters in T2 DM mice.Finally,naringenin has beneficial effects on glucose regulation in T2 DM mice.