LncRNA SNHG20 Regulated By C-MYC Promoted Diffuse Large B Cell Lymphoma Progression

Background: Diffuse large B lymphoma(DLBCL)is the most common subtype of non-Hodgkin’s lymphoma.The current standard therapy for the treatment of DLBCL was R-CHOP(cyclophosphamide,adriamycin,vincristine,prednisone and rituximab)regimen.Most DLBCL patients can be cured or achieve remission,but 40% DLBCL patient occurred hard to cure or relapse after remission.Therefore,it’s urgent to have deeper understanding for the pathogenesis of DLBCL.It has been shown that long non-coding RNA(lnc RNA)played vital roles in the tumorigenesis of DLBCL.Long non-coding RNA SNHG20(lnc RNA SNHG20)was first reported in hepatocellular carcinoma and has been shown to play an oncogene role in many cancers.However,the function of lnc RNA SNHG20 in DLBCL had never been reported yet.Our research focused on exploring whether lnc RNA SNHG20 played oncogene role in the progression of DLBCL,and the underlying mechanism in the tumorigenesis of DLBCL.Methods: 1.The GEPIA database was used to analyze the expression level of lnc RNA SNHG20 in DLBCL.2.We collected and analyzed the difference of lnc RNA SNHG20 expression between reactive lymph node hyperplasia(RLH)tissue and clinical tissue of patients with DLBCL.3.The RT-q PCR was used to analyze the lnc RNA SNHG20 expression between DLBCL cell lines(U2932,OCI-LY3,OCI-LY8,OCI-LY10)and normal B cell GM12878.4.Stably transduced U2932 cells were constructed by sh SNHG20 lentivirus.5.The CCK-8 assay was used to confirm the impact of silencing lnc RNA SNHG20 on the proliferation phenotype of DLBCL in U2932 cells.6.The flow cytometry apoptosis assay and western blotting assay were used to assess the apoptosis ability between sh SNHG20 cells and sh NC cells.7.The flow cytometry cell cycle assay and western blotting assay were used to assess the cell cycle ability between sh SNHG20 cells and sh NC cells.8.Stably transduced U2932 cells were constructed by lnc RNA SNHG20 overexpression lentivirus.9.The CCK-8 assay was used to confirm the impact of overexpression lnc RNA SNHG20 on the proliferation phenotype of DLBCL.10.The flow cytometry cell apoptosis assay and western blotting assay were used to assess the apoptosis ability between lnc RNA SNHG20 overexpression cells and NC cells.11.The flow cytometry cycle assay and western blotting assay were used to assess the cell cycle ability between lnc RNA SNHG20 overexpression cells and NC cells.12.The GEPIA was used to explore the relationship between MYC and lnc RNA SNHG20 in DLBCL.13.The efficiency of c-MYC silencing induced by small interfering RNA(si RNA)were examined by western blotting assay and RT-q PCR in U2932 cells.14.The RT-q PCR experiment was used to verify lnc RNA SNHG20 expression level after the silencing of c-MYC in U2932 cells.15.The efficiency of c-MYC overexpression induced by lentivirus were examined by western blotting assay and RT-q PCR in U2932 cells.16.The RT-q PCR experiment was used to verify lnc RNA SNHG20 expression level after the overexpression of c-MYC in U2932 cells.Results: 1.Lnc RNA SNHG20 was highly expressed in DLBCL tissues and cell lines.2.The CCK-8 assay results showed that silencing lnc RNA SNHG20 inhibited the proliferation ability in U2932 cells.3.The flow cytometry apoptosis assay as well as western blotting assay showed that silencing lnc RNA SNHG20 promoted apoptosis ability in U2932 cells.4.The flow cytometry cell cycle assay and western blotting assay showed that silencing lnc RNA SNHG20 blocked cell cycle progression.5.The CCK-8assay results confirmed that overexpression of lnc RNA SNHG20 promoted the proliferation ability of DLBCL cells.6.The flow cytometry cell apoptosis assay and western blotting assay results showed that overexpression of lnc RNA SNHG20 inhibited the apoptosis ability of DLBCL cells.7.The flow cytometry cell cycle assay and western blotting assay results showed that overexpression of lnc RNA SNHG20 promoted the progression of DLBCL cell cycle progression.8.The GEPIA database,western blotting assay and RT-q PCR assay confirmed that lnc RNA SNHG20 was regulated by c-MYC in DLBCL.Conclusion: 1.Lnc RNA SNHG20 was highly expressed in DLBCL tissues and cell lines.2.The silencing of lnc RNA SNHG20 inhibited cell proliferation,promoted apoptosis and blocked cell cycle progression in DLBCL.3.Overexpression of lnc RNA SNHG20 in DLBCL cell lines promoted cell proliferation,inhibited apoptosis and accelerated cell cycle progression.4.C-MYC regulated lnc RNA SNHG20 expression in DLBCL.Significance: This study elucidated a new molecular mechanism of malignant progression in DLBCL,which suggested that lnc RNA SNHG20 may be a potential target for the diagnosis and treatment of DLBCL in the future.