Correlation Between HMMR SNPs And BAFF And CIN Occurrence Of Breast Cancer

Objective In this study,the single nucleotide polymorphism(SNP)of hyaluronic acid mediated motion receptor(HMMR)was detected.SNP)genotypes(rs299293,rs299313,rs299314)and B-cell activation factor(BAFF)protein levels were expressed,and the correlation between them and chemotherapy-induced neutropenia(CIN)in breast cancer patients was analyzed,and the relationship between BAFF and the clinical characteristics of patients was also analyzed.Methods 1.From January 2022 to December 2022,60 breast cancer patients with advanced first-line or adjuvant chemotherapy and chemotherapy regimen containing anthracyclines and/or taxanes were selected.Before chemotherapy,blood samples were taken from anticoagulation tubes for detection of HMMR SNPs genotype and BAFF protein level.After chemotherapy,patients were divided into two groups according to whether CIN occurred or not.The group with CIN was CIN group(N1=30)and the group without CIN was the control group(N2=30).The general clinical features such as age,weight,menstrual state,primary focus and lymph node metastasis were recorded,and whether the expression of HMMR SNPs genotype and BAFF protein was related to the clinical features was analyzed.2.Process blood samples to extract RNA and detect the purity of products.The quantitative experiment of tumor necrosis factor superfamily member 13b(TNFSF13B)m RNA was carried out by PCR,and the data were recorded.3.Use ELISA to detect the expression level of BAFF in serum and record the data.4.Extract DNA from whole blood samples,conduct DNA quality control and design primer sequences,and detect the genotypes of HMMR SNPs(rs299293,rs299313,rs299314)by Tap Man probe method and record them.5.All data were processed using the SPSS 26.0 statistical software analysis package,and quantitative data were expressed as means and their standard deviations.The between-group comparisons were conducted using a t-test,and the results were expressed as mean±standard deviation.The qualitative data were analyzed with numbers(n),X~2test or Fisher exact probability method,and P<0.05 The difference was considered statistically significant.The three SNP genotypes were evaluated by X~2test with odds ratio(OR)and 95%confidence intervals to assess the association between rs299293,rs299313,rs299314 and CIN in breast cancer patients,and P<0.05 was statistically significant.Multivariate analysis using Logistic regression curve and receiver operating characteristic curve(ROC)was used,and P<0.05 indicated that it was statistically significant.Results 1.The expression of TNFSF13B m RNA in blood of patients with breast cancer before chemotherapy was found to be significantly higher in CIN group than in control group(1.5970 0.4391 vs 1.1127 0.2551,P<0.05).The expression of serum BAFF protein before chemotherapy showed that the expression of BAFF in CIN group was higher than that in control group(3445.8443 307.3805 vs 2987.8662 273.0238),and the difference was statistically significant(P<0.05).2.Logistic regression analysis was used to analyze the influencing factors affecting CIN in patients.Result prompt,high expression of BAFF protein was an independent risk factor affecting CIN(P=0.040 OR=1.003 95%C.I.:1.000～1.006).The ROC analysis results showed that the region below the ROC curve(AUC)was 0.680,P=0.043,the sensitivity was 46.7%,and the specificity was 93.6%.The area under the curve was close to 0.7,suggesting that BAFF protein could be used as a predictor of CIN in breast cancer patients.3.All 60 patients were female.There was no correlation between the age,characteristics,number of lymph node metastasis,location of primary focus and menstrual state and the expression of BAFF protein,and there was no statistical significance(P<0.05).4.The genotypes of SNP rs299293 in CIN group were 29 CC homozygotes and 1 TT homozygote,while those in control group were 28 CC homozygotes and 2 TT homozygotes.OR=2.071(95%CI 0.178～24.148,P>0.05)suggested that rs299293 was a risk factor for CIN in breast cancer,but the difference was not statistically significant.The genotypes of SNP rs299313 in CIN group were 29 GG homozygotes and 1 AA homozygotes,while in control group,29 GG homozygotes and 1 AA homozygotes,OR=1(95%CI 0.060～16.763,P>0.05)RS 299313 had no correlation with CIN of breast cancer,and the difference was not statistically significant.In CIN group,SNP rs299314 genotype was 29 TT homozygotes and 1CC homozygote.The control group consisted of 29 TT homozygotes and 1 CC homozygote.OR=1(95%CI 0.060～16.763,P>0.05)suggested that there was no correlation between rs299314 and CIN of breast cancer,and the difference was not statistically significant.Conclusion 1.The expression of TNFSF13B m RNA in the blood and the increase of BAFF protein level in serum were associated with CIN in breast cancer patients.The high expression of BAFF protein is an independent risk factor affecting the development of CIN in patients,and BAFF protein is expected to be a predictor of CIN in breast cancer patients.2.The age,weight,number of lymph node metastasis,menstrual status and the location of primary lesions of breast cancer patients have no correlation with the expression of HMMR SNPs genotype and BAFF protein level.3.The results showed that the three genotypes of rs299313,rs299293 and rs299314 were not correlated with CIN in breast cancer.