| Objective: By measuring the lung function of rats with acquired pectus excavatum before and after Nuss operation,the changes of respiratory function after the formation of pectus excavatum and the effect of Nuss operation on long-term respiratory function were discussed.Methods:Ninety four-week-old SD rats were randomly divided into control group(30rats),PE group(30 rats)and Nuss group(30 rats).The control group did not undergo any treatment.The PE group and the Nuss group made funnel chest models by cutting off the 5th,6th and 7th pairs of costal cartilages beside the sternum.The PE group only made models.The Nuss group used Z shaped steel needles to perform Nuss surgery 4 weeks after the model was made,and the internal fixation steel needles were taken out 4 weeks after the orthopedic operation.The lung function was measured and compared between groups at 4 weeks,8weeks and 12 weeks after the removal of internal fixation after the Nuss operation.Results:(1)4 weeks after removal of internal fixation by mimicking Nuss surgery:(1)Comparison between control group and PE group: the difference between FEF5016.57(15.56-17.52)in PE group rats compared to 21.40(20.34-22.77)in control group was significant(P < 0.05);FEF75 13.47(13.21-14.29)compared to 17.76(16.19-19.54)in control group was significant(P < 0.05);PEF23.59(23.02-24.07)compared to control 25.45(24.81-26.38)(P < 0.05);Rl,Re,Cdyn,FVC,FEV1,FEV1/FVC were not statistically significant compared to control(P > 0.05).(2)Comparison between control group and imitation Nuss group: Cdyn 0.22(0.19-0.23)in imitation Nuss group rats compared with 0.23(0.22-0.24)in control group was significant(P < 0.05);FVC 6.03(4.87-6.74)compared with 9.07(8.66-9.75)in control group was significant(P < 0.05);FEV1/FVC 63.02(57.57-73.01)compared with 64.06(60.51-65.24)in the control group was significant(P < 0.05);FEF50 20.32(19.10-21.39)compared with 21.40(20.34-22.77)in the control group was significant(P < 0.05);FEF7516.57(14.40-17.77)was significant(P < 0.05)compared with 17.76(16.19-19.54)in the control group;Rl,Re,FEV1,and PEF were not statistically significant(P > 0.05)compared with the control group.(3)Imitation Nuss group compared with PE group: the difference was significant(P< 0.05)between Rl 0.49(0.46-0.53)in the Imitation Nuss group rats compared with 0.50(0.48-0.53)in the model group;Re 0.31(0.30-0.32)compared with 0.55(0.44-0.57)in the model group(P < 0.05);Cdyn 0.22(0.19-0.23)was significant(P < 0.05)compared to 0.21(0.18-0.23)in the model group;FEF50 20.32(19.10-21.39)was significant(P < 0.05)compared to 16.57(15.56-17.52)in the model group;FEF75 16.57(14.40-17.77)compared with 13.47(13.21-14.29)in the model group was significant(P < 0.05);FVC,FEV1,FEV1/FVC,and PEF were not statistically significant compared with the model group(P >0.05).(2)Eight weeks after removal of internal fixation by mock-Nuss surgery:(1)Control group compared with PE group: Rl 0.51(0.49-0.53)in model group rats compared with 0.45(0.42-0.48)in control group was significant(P < 0.05);Re 0.31(0.22-0.36)compared with 0.30(0.28-0.43)in control group was significant(P < 0.05);Cdyn0.20(0.16-0.27)compared to 0.23(0.22-0.25)in the control group(P < 0.05);FVC 5.46(4.19-6.29)compared to 7.68(7.14-7.93)in the control group(P < 0.05);FEV 13.05(2.00-4.08)compared to 5.53(5.45-5.54)in the control group(P < 0.05).5.45-5.54)compared with the control group;FEV1/FVC 48.80(40.16-52.63)compared with 72.01(69.86-76.33)compared with the control group;FEF50 16.49(15.89-17.63)compared with 20.09(19.63-20.75)compared with the control group.-(P < 0.05);FEF75 11.86(11.32-12.84)compared to control 16.68(15.54-18.50)(P < 0.05);PEF 23.49(22.89-23.70)compared to control 24.14(23.07-24.52)The difference was significant(P < 0.05)compared to the control group 24.14(23.07-24.52).(2)The difference between the control group and the imitation Nuss group was significant(P < 0.05): FEV1 3.52(3.51-3.54)in the imitation Nuss group compared with 5.53(5.45-5.54)in the control group;FEV1/FVC 55.87(50.07-58.79)compared with 72.01(69.86-76.33)in the control group.(P < 0.05);FEF50 21.09(20.59-21.80)compared to control 20.09(19.63-20.75)(P < 0.05);FEF75 18.02(17.44-19.26)compared to control 16.68(15.54-18.50)(P < 0.05);PEF 26.34(26.25-26.50)compared with 24.14(23.07-24.52)in the control group was significant(P < 0.05);Rl,Re,Cdyn,and FVC were not statistically significant(P > 0.05)compared with the control group.(3)Imitation Nuss group compared with PE group: the difference between Rl 0.45(0.38-0.52)in the Imitation Nuss group rats compared with 0.51(0.49-0.53)in the model group was significant(P < 0.05);the difference between Re 0.29(0.26-0.28)compared with0.31(0.22-0.36)in the model group was significant(P < 0.05);Cdyn 0.23(0.20-0.26)compared to 0.20(0.16-0.27)in the model group(P < 0.05);FVC 6.30(5.97-7.07)compared to 5.46(4.19-6.29)in the model group(P < 0.05);FEV1 3.52(3.51-3.54)compared to(P <0.05);FEV1/FVC 55.87(50.07-58.79)compared to 48.80(40.16-52.63)in the model group;FEF50 21.09(20.59-21.80)compared to 16.49(15.89-21.80)in the model group.16.49(15.89-17.63)compared to the model group(P < 0.05);FEF75 18.02(17.44-19.26)compared to the model group 11.86(11.32-12.84)(P < 0.05);PEF 26.34(26.25-26.50)compared to the model group 23.49(PEF 26.34(26.25-26.50)compared with 23.49(22.89-23.70)in the model group was significant(P < 0.05).(3)12 weeks after removal of internal fixation by mock-Nuss surgery:(1)Control group compared with PE group: significant difference(P < 0.05)in Re0.31(0.30-0.42)in PE group rats compared with 0.30(0.29-0.31)in control group;significant difference(P < 0.05)in FVC 5.25(4.79-5.94)compared with 7.33(6.62-7.96)in control group;FEV1 2.15(1.01-2.57)compared with 5.49(5.29-5.55)in the control group was significant(P < 0.05);FEV1/FVC 39.38(20.20-41.79)compared with 74.90(69.23-79.91)in the control group was significant(P < 0.05);FEF50 15.70(15.00-16.98)compared to 18.92(17.73-20.63)in the control group(P < 0.05);FEF75 11.21(10.61-15.55)compared to 14.49(12.85-16.81)in the control group(P < 0.05);PEF 21.10(20.00-24.21)compared to 23.75(22.50-24.89)in the control group was significant(P < 0.05);Rl and Cdyn were not statistically significant compared to the control group(P > 0.05).(2)Control group compared with imitation Nuss group: the difference between Rl0.45(0.42-0.49)in the imitation Nuss group rats compared with 0.51(0.45-0.53)in the control group was significant(P < 0.05);the difference between Cdyn 0.25(0.23-0.27)compared with 0.20(0.20-0.24)in the control group was significant(P < 0.05);The differences in Re,FVC,FEV1,FEV1/FVC,FEF50,FEF75 and PEF were not statistically significant(P > 0.05)compared with the control group.(3)Comparison between the mock-Nuss group and PE group: Rl 0.45(0.42-0.49)in rats in the mock-Nuss group compared with 0.53(0.48-0.56)in the model group was significant(P < 0.05);Re 0.28(0.27-0.30)compared with 0.31(0.30-0.42)in the model group was significant(P < 0.05);Cdyn 0.25(0.23-0.27)compared to 0.19(0.19-0.23)in the model group was significant(P < 0.05);FEV1/FVC 52.58(43.52-64.37)compared to 39.38(20.20-41.79)in the model group was significant(P < 0.05);FEF75 14.23(12.32-19.99)compared to 11.21(10.61-15.55)in the model group(P < 0.05);FVC,FEV1,FEF50,and PEF were not statistically significant compared to the model group(P > 0.05).Conclusion:1.After correcting the thoracic deformity of funnel chest rats by mimicking Nuss surgery,the respiratory function improved with the removal of internal fixation and time.2.Long-term respiratory function improvement was close to normal. |
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