Effect Of MiRNA-21-5p On BCG Induced Macrophage Immune Regulation Via PI3K/AKT Signaling Pathway

Objective: To explore the role of miRNA-21-5p and PI3K/AKT signaling pathway in BCG induced macrophage immune regulation by establishing a cell model.Methods:Macrophages were infected by BCG,and four model groups were established,namely TB group,TB+miRNA21 group,TB+LY group and TB+miRNA21+LY group,and a separate negative control group was established using only DMOS culture.The expression levels of miRNA-21-5p and pathway-related genes PI3 K and AKT were detected by q RT-PCR.Results:The expression level of miRNA-21-5p in TB group,TB+miRNA21 group and TB+miRNA21+LY group was significantly decreased at 12 h time point compared with 0h time point(P<0.05).The expression level of AKT in TB group and TB+miRNA21 group was significantly increased at 6h and 12 h time points compared with 0h time points(P<0.05),and the expression level of AKT in TB group was significantly increased at 12 h time points compared with 6h time points(P<0.05).The expression level of PI3 K in TB+miRNA21 group was significantly increased at 12 h,24h and 48 h time points compared with 0h time points(P<0.05).The expression of miRNA-21-5p in TB+miRNA21 group and TB+miRNA21+LY group at 12 h time point was significantly decreased compared with DMOS group at the same time point(P<0.05).AKT expression level in TB group was significantly increased at 12 h time point(P<0.05).PI3 K in TB+miRNA21 group and TB+miRNA21+LY group was significantly increased at 12 h time point(P<0.05),and PI3 K in TB+miRNA21 group was significantly increased at 24 h time point(P<0.05).Conclusions: When BCG infects macrophages,the secretion of miRNA-21-5p is down-regulated,and the PI3K/AKT signaling pathway is negatively regulated,thus inducing macrophages to perform immune regulation.