MiRNA-141 Participates In The Pathogenesis Of Preeclampsia By Regulating Trophoblast Apoptosis Through PI3K/Akt Pathway

Research PurposesIn this study,the expression of mir NA-141 in cultured trophoblast Bewo cells in vitro was regulated to study its effect on trophoblast cell apoptosis and explore the possible pathogenesis of mir NA-141 in preeclampsia Experiment Content1.Trophoblast cell line Bewo cells were transfected with miR-141 overexpressed plasmid,silenced plasmid and negative control plasmid,respectively.48 h after transfection,apoptosis rate of cells was detected by flow cytometry to verify the effect of Mir-141 on trophoblast cell apoptosis.2.After plasmid grouping transfection,the expression levels of PI3 K and Akt protein in the trophoblast cell line Bewo cells were detected by Western blot,and the gray values of each band were measured and analyzed by Image J software to observe the effect of miR-141 on the expression of PI3 K and Akt in Bewo cells.Experiment Results1.Compared with the negative control group（P=0.021）and blank control group（P=0.032）,the apoptosis rate of Bewo cells in miR-141 overexpressed plasmid transfection group was decreased.There was no significant difference in apoptosis rate between the negative control group and the blank control group（P=0.444）.2.The apoptosis rate of Bewo cells transfected with miR-141 silencing plasmid was higher than that of the negative control group（P=0.0099）and blank control group（P=0.059）;There was no significant difference in apoptosis rate between the negative control group and the blank control group（P=0.444）.3.The expression of PI3 K protein in miR-141 overexpressed plasmid transfection group was higher than that in the negative control group（P= 0.024）and the blank control group（P= 0.033）,and the PI3 K protein level in the silent plasmid transfection group was lower than that in the blank control group（P < 0.001）and the negative control group（P=0.0043）.There was no significant difference in PI3 K expression between blank control group and negative control group（P > 0.05）.4.The expression level of Akt protein in miR-141 overexpression group was higher than that in the negative control group（P=0.0053）and blank control group（P=0.0007）,and the level of Akt protein in the silent group was lower than that in the blank group（P=0.038）and blank control group（P=0.0012）.There was no significant difference between blank control group and negative control group（P > 0.05）.Experiment ConclusionmiRNA-141 can regulate the apoptosis of trophoblast cells through the intracellular PI3K/Akt pathway and participate in the pathogenesis of preeclampsia.