| Objectives:1.to screen genes recognized to be closely related to the development of coronary heart disease at home and abroad through systematic evaluation;2.to explore the correlation between coronary heart disease-related genes and the prevalence of coronary heart disease in hospitalized patients in Hainan,and to provide a theoretical basis for enriching the screening tools and optimizing the prevention and treatment strategies for high-risk groups of coronary heart disease in Hainan;3.to explore the correlation between genes at high risk of coronary heart disease and patients’ blood lipid levels,history of smoking,history of alcohol consumption,history of hypertension,history of diabetes mellitus and age.Methods:1.A total of 22 genes that may be associated with coronary heart disease were initially established by searching domestic and international databases and literature study,and then Pub Med,CNKI,Wanfang database and SCIE database were systematically searched,duplicates were excluded,the frequency of these genes being reported was statistically analyzed,and 50% of them were selected as the ones judged to be relatively more frequently reported at home and abroad in relation to coronary heart disease The genes with relatively close relationship with coronary heart disease were selected,and the genes with high correlation with the development of coronary heart disease were initially screened out and reported in many studies at home and abroad as the basis for further research;2.Inpatients attending the Second Affiliated Hospital of Hainan Medical College from 1 January 2020 to 30 June 2022,adults of Hainan origin and aged >30 years were selected,and the patients were divided into coronary heart disease group and non-coronary heart disease group.Gene expression was quantified using PCR real-time fluorescence,and the correlation between coronary heart disease gene expression and coronary heart disease was explored using spearman correlation analysis,multi-factor logistic regression analysis,and plotting ROC curves;to explore whether there were ethnic differences(Han vs.Li)in the inpatients with high expression of coronary heart disease genes;3.pearson correlation analysis was used to explore the correlation between highly expressed genes in the coronary heart disease group and blood lipid index,history of smoking,history of alcohol consumption,history of hypertension,history of diabetes mellitus,and age.Results:1.After this Pub Med,CNKI,Wanfang,and SCIE database literature search a total of 36538 articles were retrieved,excluding 3323 articles with duplicate reports,leaving 33215 articles.4542 articles related to the study objectives were screened by reading the titles and abstracts,excluding 3301 articles and finally including 1241 articles.Among them,there are currently 11 genes with more than 50 reported articles,including APOE,APOB,AGT,ACE,GNB3,MTHFR,NOS3,ESLE,IL-6,TNF and FGB.2.The number of cases included in this study was higher in the coronary group(170)than in the non-coronary group(55).In terms of gender,the number of men in the coronary group(126)was higher than the number of women in the non-coronary group(26)and the number of women in the non-coronary group(26)was lower than the number of men(29),and there was a difference in gender between the two groups,P=0.03,P<0.05.Apolipoprotein B gene(Apo B)was significantly more highly expressed in the coronary group,P<0.0001;the AGT gene(P=0.0158),Apo E gene(P=0.0126),FGB gene(P=0.005),GNB3 gene(P=0.0151),MTFHR gene(P=0.0119),SELE gene(P=0.005),TNF gene(P= 0.0298)were significantly highly expressed in the non-coronary group,and the expression of NOS3 gene(P=0.3047),IL6 gene(P=0.7239),and ACE gene(P=0.7852)did not differ between the two groups.In correlation analysis,coronary artery disease was negatively correlated with the AGT gene(rs=-0.163,p=0.011,p<0.05),the APOB gene(rs=0.75,p=0,p<0.01),the FGB gene(rs=-0.163,p=0.011,p<0.05)and the GNB3 gene(rs=-0.165,p=0.011,p<0.05),SELE gene was negatively correlated(rs=-0.171,p=0.007,p<0.01),MHTHR gene was negatively correlated(rs=-0.210,p=0.001,p<0.01),TNF gene was negatively correlated(rs=-0.131,p=0.04,p<0.05 Logistic regression analysis showed that the risk of coronary heart disease was 2.298 times higher in patients with high Apo B gene expression than in those without high Apo B gene expression,and the ROC curve area(AUC=0.989)was greater than 0.7,indicating that the Apo B gene has a strong predictive value for the development of coronary heart disease.The expression of APOB,MHTHR,ACE,APOE,FGB,SELE,AGT,TNF,NOS3,IL6 and GNB genes was not statistically significant in the Han and Li populations in Hainan,P>0.05.3.The Apo B gene for coronary heart disease was negatively correlated with triglycerides,with a correlation coefficient(rs=-0.461,p=0),positively correlated with age,with a correlation coefficient(rs=0.173,p=0.009),positively correlated with total cholesterol,with a correlation coefficient(rs=0.499,p=0),negatively correlated with HDL,with a correlation coefficient(rs=-0.181 Apo B gene was significantly and positively correlated with history of smoking(r=0.193,p<0.004)and diabetes(r=0.135,p=0.045);it was not significantly correlated with history of alcohol consumption,family history of coronary heart disease,history of hypertension,or age,p>0.045.No significant correlation was found with history of alcohol consumption,family history of coronary heart disease,history of hypertension and age,P > 0.05.Conclusions:1.This part of the study suggests that APOE,APOB,AGT,ACE,MTHFR,NOS3,ESLE,IL-6,TNF,FGB,GNB3 genes are highly correlated with the development of coronary heart disease,which lays the foundation for further related studies.2.Apolipoprotein B gene may be a high-risk gene for developing coronary heart disease in Hainan population,which may enrich the screening tools for the high-risk group of coronary heart disease in Hainan and may improve our current prevention strategy.3.The genes AGT,Apo E,FGB,GNB,MTFHR,SELE and TNF may be protective genes in the Hainan population for coronary heart disease,and high expression of these genes may reduce the incidence of coronary heart disease,but further studies are needed.4.In Hainan population,the expression of apolipoprotein B gene was associated with lipid metabolism,diabetes mellitus and smoking,which may indirectly affect the occurrence of coronary heart disease through lipid metabolism,but further in-depth study is still needed. |
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