The Effect Of AHMX6 On The Proliferation Of SGC7901 Human Gastric Cancer Cells And Its Mechanism

Objective: The purpose of this study is to investigate the inhibitory effect of AHMX6,a novel tubulin inhibitor,on the proliferation of human gastric cancer cell SGC7901 and its mechanism.Methods: We measured the effect of the drug on proliferation of SGC7901 cells using the CCK-8 method;Flow cytometry was used to assess the effect of PI-stained cells on cell cycle,and Western blot analysis was used to monitor cell cycle related protein expression in SGC7901 gastric cancer cells.Results: 1.Human gastric cancer cells SGC7901 were shown to be inhibited by AHMX6 compound in this study,and the inhibitory effect on cell proliferation was time-dependent and dose-dependent,and there was a difference between groups(P<0.05 or P<0.01).2.The drug AHMX6 is capable of causing G2/M cycle arrest in SGC7901 cells,and the proportion of cells in G2/M phase increases with increasing drug concentrations.A statistically significant difference exists between the groups compared to the control group.3.Western blot showed that the expression level of Cdc25 c and cyclin A protein in SGC7901 cells treated with this drug was significantly lower than that in the control group,p21 protein expression was significantly higher than in the control group.The difference between the two groups was statistically significant;CDK1 and cyclin B1 did not change significantly,and there was no statistical significance.Conclusion: 1.AHMX6 can inhibit the proliferation of gastric cancer SGC7901 cells.2.AHMX6 can induce SGC7901 cell cycle arrest in G2/M phase.3.AHMX6 inhibits the proliferation of gastric cancer SGC7901 cells and induces G2/M cycle arrest,which may be related to down-regulating the expression of Cdc25 c and cyclin A proteins in gastric cancer SGC7901 cells and up-regulating the expression of p21 protein in gastric cancer SGC7901 cells.