Anti?inflammation Properties Of Resveratrol In The Detrusor Smooth Muscle Of The Diabetic Rat

Objective: To prove that resveratrol may treat diabetic cystopathy(DCP)by regulating the systemic inflammatory response.Methods: The Sprague-Dawley(SD)rat model of type 1 diabetes was induced by intraperitoneal injection of streptozotocin(STZ).Then separated SD rats into four groups:(1)excipient-treated control group;(2)resveratrol-treated control group;(3)excipient-treated streptozocin(STZ)injected group;(4)resveratrol-treated STZ injected group.Resveratrol or excipient was administered by intragastric administration.After twelve weeks of diabetes induction,blood sugar concentrations and bladder weights were measured and the bladder tissue of each group of rats was taken for hematoxylin-eosin staining to observe histological changes,Real-time Quantitative polymerase chain reaction(q PCR),and Western blotting to analyze the levels of tumor necrosis factor-alpha(TNF-α),nuclear factor kappa B(NF-κB),interleukin(IL)-6,and IL-1β expression.Results: The bodyweight of diabetic rats was appreciably reduced,while the bladder weight and blood glucose concentrations were significantly increased.Oral resveratrol could not improve the changes in bodyweight and blood glucose concentrations but had a certain effect on bladder weight.The macroscopic evaluation evinced that the bladder wall of STZ-induced diabetes rats was evidently thickened,and HE staining presented that the bladder tissues of diabetes rats had inflammatory cell infiltration,edema,and capillary congestion of the mucosa and lamina propria.After resveratrol treatment,bladder wall thickening was reduced,and tissue damage and inflammation were significantly ameliorated.All these changes were associated with markedly heightened expressions of TNF-α,IL-1β,IL-6,and NF-κB in detrusor smooth muscle(DSM)tissues of diabetic rats.Oral treatment with resveratrol alleviated the expressivity of inflammatory cytokines in DSM tissues.Conclusion: Resveratrol treatment ameliorated the histological changes in the bladder and inhibits the expression of DSM tissue inflammatory factors in diabetes rats.Resveratrol may supply a new area for the treatment of diabetic cystopathy.