Comparative Analysis Of Oral Salivary Microbiome In Patients With Liver Cirrhosis And Normal Population

Objective: To compare and analyze the differences in the abundance and diversity of oral salivary microbiome between liver cirrhosis patients(LC group)and normal population(N group),and to clarify the dominant bacterial flora in oral cavity of patients with liver cirrhosis.Methods: The oral saliva of 45 liver cirrhosis patients and 44 healthy individuals were collected and analyzed by high-throughput sequencing technology.The ASVs were obtained after combining and qualifying of the raw tags,and then bioinformatics analysis including species annotation,diversity analysis and LEf Se analysis were performed.Results: A total of 6314 ASVs were obtained in this study,including 3224 in the liver cirrhosis patients,4300 in the normal population,and the total number of ASVs in both groups was 1210.Species Accumulation Boxplot indicated that the sample was sufficient and there was no significant difference in Alpha diversity index between the two groups(all P>0.05).The difference of oral salivary flora structure between liver cirrhosis patients and normal population was statistically significant(F=20.13,P<0.05),and the difference of community structure between groups was significantly greater than that within groups(R>0,P<0.05).LEf Se analysis results showed that the relative abundance ratio of 19 kinds of bacteria such as Firmicutes,Bacteroidetes and Fusobacteria in the oral cavity of patients with liver cirrhosis was higher.However,the relative abundance ratio of 8 species including Proteobacteria,Pasteurellales and Neisseria in the oral cavity of normal population was relatively high.Conclusion: Although the composition of dominant bacteria in oral saliva was basically the same between liver cirrhosis patients and normal population,but the structure of bacteria was significantly different.Liver cirrhosis patients with oral flora structure disorders are more likely to suffer from caries and periodontal diseases than the normal population.It is important to pay attention to oral health and prevent the imbalance of oral flora in the diagnosis and treatment of liver cirrhosis...