Value Of Urinary Alzheimer’s-associated Neurofilament Protein Combined With Serum Inflammatory Factors In The Diagnosis Of Alzheimer’s Disease

Objective: To investigate whether urine Alzheimer’s associated neurofilament protein(AD7c-NTP)alone is more advantageous in the diagnosis of Alzheimer’s disease(AD)than combined with interleukin-6(IL-6)and high-sensitivity C-reactive protein(Hs-CRP).Methods: 64 AD patients admitted to our hospital in the past three years were selected as the case group,and a control group of 64 healthy physical examinees who visited our hospital was also selected.The urine AD7c-NTP,serum IL-6,and serum Hs-CRP levels of both groups were tested,and the Mini-Mental State Examination(MMSE)questionnaire was completed.The case group was also evaluated with the Clinical Dementia Rating(CDR)questionnaire.The basic characteristics of the two groups were compared,and the results of serum IL-6,serum Hs-CRP,and urine AD7c-NTP levels were compared.The correlation between MMSE scores and serum IL-6,serum Hs-CRP,and urine AD7c-NTP levels in the case group were analyzed.The diagnostic value of urine AD7c-NTP alone and combined with serum inflammatory factors detection for AD patients was analyzed,using ROC curve to analyse.Results:Compared with the urine AD7c-NTP,serum IL-6,and serum Hs-CRP test results of the control group,both mild and moderate to severe AD patients had significant differences(all P<0.05).Through analysis,it was found that the urine AD7c-NTP,serum IL-6,and serum Hs-CRP levels of AD patients were negatively correlated with MMSE scores(all r<0),and the former two levels were positively correlated with the patient’s CDR score(both r>0).The urine AD7c-NTP level of AD patients was positively correlated with their serum IL-6 and serum Hs-CRP levels(r>0).Logistic regression analysis showed that the sensitivity and specificity of multi-index joint detection for the diagnosis of AD in AD patients were 76.6% and 96.9%,respectively,and the AUC curve area was 0.935.ROC curve analysis showed that the difference between the three indicators detected alone and in combination was statistically significant(P<0.05).Conclusion: The levels of AD7c-NTP in urine,IL-6 in serum,and Hs-CRP in serum of case group were significantly higher compared to the control group.The levels of AD7c-NTP in urine,IL-6 in serum,and Hs-CRP in serum of case group were negatively correlated with their cognitive level,while the levels of AD7c-NTP in urine and IL-6 in serum were positively correlated with the severity of the disease.The joint detection of AD7c-NTP in urine,IL-6 and Hs-CRP in serum has good diagnostic value,and the combination of these three tests is more suitable for further testing of suspected AD patients after initial screening.