Salidroside Hydrogel For The Treatment Of Atopic Dermatitis In Mice

Atopic dermatitis(AD)is a common disease with a genetic predisposition to recurrence and is difficult to cure completely;there are two main types of AD,endogenous AD and exogenous AD.The most distinctive feature of exogenous AD is a high level of immunoglobulin E(Ig E),which is usually associated with severe pruritus and is often accompanied by other diseases such as allergic rhinitis and asthma.Currently,glucocorticoids and antihistamines are the main drugs used to treat AD,but glucocorticoids are often ineffective in treating patients with moderate to severe AD,and their prolonged use can cause significant side effects.For example,the long-term use of glucocorticoids in children may have a negative impact on their growth and development.The clinical evaluation of the degree of skin damage in atopic dermatitis is usually based on two evaluation methods: EASI(Eczema are and severity index)or SCORAD(Scoring Atopic Dermatitis index).However,both of these methods are semi-quantitative and subjective in nature,and require a certain level of experience on the part of the clinician in order to score the lesion,which makes the data obtained somewhat subjective.However,NIR two-zone fluorescence imaging can indicate the degree of AD skin lesion based on the intensity of the fluorescence signal at the skin lesion and thus can quantify the degree of AD skin lesion.Therefore,we first attempted to introduce NIR two-zone fluorescence imaging into the evaluation of the extent of atopic dermatitis lesions.Hydrogel is a class of polymers consisting of hydrophilic polymer chains with a three-dimensional mesh structure.It has many excellent properties,such as good cytocompatibility and low toxicity.These excellent properties determine that hydrogel materials are widely used in biomedical engineering and tissue engineering.It has been reported that Rhodiola rosea has certain anti-inflammatory,antioxidant,anti-tumor and antifungal effects,not only that Rhodiola rosea has some efficacy in the treatment of immune diseases,diabetes and cardiovascular diseases.Based on the above research,we tried to use hydrogel-encapsulated Tibetan medicine rhodiola glycosides for the treatment of atopic dermatitis.The quaternized β-chitosan hydrogel of rhodiroside used in this study was used for treatment,providing a delivery system for topical and transdermal delivery of rhodiroside.At p H 7.4,the cumulative release of rhodiarome glucoside could reach 82 % at 72 h.The in vitro release indicated that QCOD@Sal(rhodiarome glucoside hydrogel)has a good slow release effect,and further studies on the effect of QCOD@Sal on atopic dermatitis in mice further revealed that QCOD@Sal could be used to treat atopic dermatitis by regulating the inflammatory factors TNF-α,IL-6 and skin ROS content to promote skin repair without skin irritation.This provides a new approach for the treatment of AD.This study is also the first to apply near infrared region Ⅱ(NIR Ⅱ,1000-1700 nm)imaging to AD scoring to monitor the treatment process and the extent of skin damage in AD in real time.These attractive results provide a new perspective for designing NIR-Ⅱ probes for NIR-Ⅱ imaging and a new approach for future clinical practice to be able to score AD objectively and guide the use of medication rationally.