Preparation And Properties Of Mussel-Inspired Hemostatic Hydrogel Adhesive

Traumatic blood loss caused by various emergencies is closely related to pre-hospital death.The body’s own coagulation process can convert flowing blood into blood clots to complete hemostasis,but it is unable to stop bleeding in time,especially in emergency situations.The commonly used methods of hemostasis in clinic are surgical sutures,gauze,electric coagulation knife,etc.,but the delicate and complex suture process takes a long time,and can not realize the rapid closure of the wound and instant hemostasis.Electric coagulation knife requires special equipment and has certain damage to human tissue.In comparison,tissue adhesive is convenient to use,fast to close the wound,fast to stop bleeding,and small damage to the tissue.Over the past few decades,various adhesive hemostatic materials have been developed,and they are reported to have the potential ability to stop bleeding during surgical procedures.However,there are drawbacks that may limit the applicability of some of these products.Commercial fibrin adhesives are too weak to be washed away in the bloodstream and carry the risk of infectious disease;Cyanoacrylates are powerful adhesives,but their degradation products are toxic and their hemostatic applications are still limited.Therefore,rapid bleeding control is still the focus of clinical attention.Hydrogels are 3D cross-linked hydrophilic polymers with a physical structure similar to that of natural extracellular matrix.They have significant characteristics,such as high porosity,biocompatibility and the ability to carry drugs.High porosity is one of the key characteristics of hemostasis,which can accelerate hemostasis by rapidly absorbing blood and absorbing red blood cells and platelets.In this study,a fast photocuring hydrogel material mimics mussel VEGF based on the double network system of methylallyl acylated gelatin and oxyglucan was prepared.Gelatin,as the main component of extracellular matrix in most tissues,contains many arginine-glycine-aspartic acid sequences that promote cell attachment,which has excellent biocompatibility.The introduction of methacrylic anhydride allows rapid photocuring of hydrogels.Adding oxyglucan to enhance mechanical properties,dopamine has certain hemostatic properties and can further enhance the adhesion of materials.Hydrogel adhesive hemostatic material has strong adhesion,drug release,biodegradability,biocompatibility and excellent hemostatic ability.The hydrogel material can be gelatinized within 15 s and adhered to the wound site to seal and stop bleeding,which can reduce the amount of bleeding in the hemostatic experiment.The adhesion of the hydrogel material to red blood cells and platelets was observed by scanning electron microscopy.The loading of VEGF into the hydrogel material can accelerate angiogenesis and promote wound healing.Compared with the control group,VEGF loaded experimental group can significantly accelerate the healing of skin defects.In addition,the hemolytic and biocompatibility of the hydrogel material meet the human implantation standards,and it also has good degradability in the mouse back skin implantation experiment,which can effectively avoid the removal of the second operation.Therefore,multifunctional hemostatic hydrogel adhesive is expected to provide some ideas for future medical hemostatic materials.