Sialic Acid And Synovial Tissue Pathological In Knee Joint Correlative Study Of Osteoarthritis

Objective: To analyze the correlation between the content of sialic acid(SA)in knee synovial fluid and the pathological score of knee synovial tissue(Krenn’s pathological score)in patients with knee osteoarthritis(KOA),and the clinical and laboratory test indexes of KOA,To further study the clinical significance of synovial SA and Krenn’s pathological score in the pathogenesis of KOA.Methods: Interleukin-6(IL-6),synovial SA,erythrocyte sedimentation rate(ESR),C-reactive protein(CRP)and tumor necrosis factor(TNF-α)in synovial fluid of 40 patients with KOA were collected.Laboratory test indicators such as the Krenn’s pathological score of knee joint synovial tissue,and clinical indicators such as age,gender,body mass index(BMI),imaging data of knee(K-L grade),knee joint function score(WOMAC).All indicators were analyzed by statistical methods such as t test,Pearson correlation coefficient,rank sum test and single factor score of variance.Results: 1.There was no statistically significant difference between pathological score and synovial SA of the knee between the Gender and the knee synovial tissue of KOA patients(P=0.129,P=0.566);2.Synovial SA level and serum CRP,serum ESR,synovial IL-6,synovial TNF-α in KOA patients.There was a positive correlation(Rs=0.154,P=0.344;Rs=0.134,P=0.378;Rs=0.812,p<0.0001;Rs=0.259,P=0.107),among which only the correlation between synovial SA and synovial IL-6 was statistically significant;3.The level of SA in synovial fluid of KOA patients was positively correlated with age and BMI(Rs=0.206,P=0.202;Rs=0.256,P=0.111).The level of SA in synovial fluid of KOA patients was positively correlated with the WOMAC score scale of knee function before treatment and the WOMAC score scale of knee function obtained from outpatient follow-up after 3 months of treatment(Rs=0.166,P=0.306;Rs=0.154,P=0.344);4.Krenn’s pathological score of knee synovial tissue and serum CRP,synovial IL-6 and synovial TNF-α in KOA patients There was a positive correlation(Rs=0.103,P=0.528;Rs=0.323,P=0.0042;Rs=0.176,P=0.278).The Krenn’s pathological score of knee synovial tissue in KOA patients was negatively correlated with serum ESR(Rs=-0.246,P=0.126).Only the Krenn’s pathological score of knee synovial tissue and the correlation between IL-6 in synovial fluid were statistically significant;5.Knee joint synovial tissue Krenn’s pathological score of KOA patients was positively correlated with age,BMI,knee joint WOMAC score scale before treatment,and knee joint WOMAC score scale obtained from outpatient follow-up after 3 months of treatment(Rs=0.589,P<0.001;Rs=0.318,P=0.046;Rs=0.260,P=0.105;Rs=0.176,P=0.278),among which the correlation between knee synovial tissue Krenn’s pathological score and patient’s age and BMI was statistically significant;6.The Krenn’s pathological score of knee synovium was positively correlated with the level of SA in synovial fluid of KOA patients(Rs=0.454,P=0.003),and the difference was statistically significant.Conclusion: 1.The pathological scores of synovial SA and Krenn’s of knee synovial tissue in KOA patients are positively correlated with the functional grade of knee joint(WOMAC)after treatment.The prognosis of KOA can be effectively predicted by measuring the pathological scores of synovial SA and Krenn’s of knee synovial tissue;2.The SA level of synovial fluid in KOA patients can reflect the degree of synovitis in KOA patients,and can be an important indicator of the development and progression of synovial inflammation in KOA patients;3.The level of SA in synovial fluid of KOA patients can be monitored at different stages of KOA,and can be used as a specific indicator of KOA to guide clinical diagnosis and treatment.