| Objective:1.To look at the existing state of PGR use in the growing of Angelica dahurica herbs and to screen out potential PGR kinds.2.To provide an analytical technique for detecting numerous PGR residues in Angelica dahurica at once.Using the established method to analyze sample Angelica dahurica,one can determine the residue levels of regulators in commercially available Angelica dahurica herbs.3.Establish the specific chromatogram of Angelica dahurica and identify the common peaks.4.The PGRs detected from Angelica dahurica samples were selected for field experiments to analyze the effect of some PGRs on the quality of Angelica dahurica by the content of compounds recognized.Methods:1.To gather the necessary samples of Angelica dahurica,to conduct research and market analysis in the tract producing area of Angelica dahurica,to conduct field study on the planting base and cultivation of Angelica dahurica herbs.2.Creation of a UPLC-MS/MS-based technique for the detection of various PGR residues in Angelica dahurica.3.Establishment of characteristic profiles of Angelica dahurica based on UPLC and identification of peaks using standards.4.Through field tests,the PGR treatment group with various concentrations and the control group with clear water application were created.Following harvest,the chemicals selected as targets were used to determine the composition of the test field samples.Results:1.Through screening,the 34 plant growth regulators that may be applied to the cultivation of Angelica dahurica were discovered.Angelica dahurica samples were acquired in 66 batches via market and origin purchases.2.34 PGR residues in Angelica dahurica were determined using a quick,precise,and concurrent analytical approach that was developed.66 batches of dahurica were gathered using this method,and 7 exogenous PGRs(4-NP,5-NG,CCC,PP333,DA-6,TBZ,and Pix)were found,with 4-NP,5-NG,CCC,and PP333 being found at a rate more than 20%.3.The UPLC specific chromatogram of Angelica dahurica herbs were constructed and nine common peaks were successfully identified using the standards,including oxypeucedanin hydrate,byakangelicin,xanthotoxin,bergapten,byakangelicol,oxypeucedanin,imperatorin,phellopterin,isoimperatorin;the similarity analysis showed that the similarity of all 10 batches of Angelica dahurica samples was higher than 0.9.4.By planning field experiments,the contents of nine coumarin-like components in the samples of Angelica dahurica applied with various amounts of PP333 and CCC were independently simulated;According to the findings,CCC considerably decreased the levels of oxypeucedanin hydrate,byakangelicin,xanthotoxin,bergapten,and byakangelicol in Angelica dahurica;Oxypeucedanin hydrate,byakangelicin,bergapten,and isoimperatorin levels increased at low PP333 concentrations but declined at higher PP333 concentrations;It was discovered that the application of CCC and a high concentration of PP333 altered the ratio of nine coumarin components in the Angelica dahurica herb.Conclusion:A method was developed for the simultaneous determination of multiple PGR residues in Angelica dahurica based on UPLC-MS/MS with 34 commonly used PGRs screened;Residue testing of 66 batches of Angelica dahurica samples using the established analytical method revealed widespread PGR residues in Angelica dahurica herbs;According to the test field samples,it was discovered that high concentrations of CCC and PP333 decreased the accumulation of various coumarin-like components in Angelica dahurica while altering the ratio between the amounts of each coumarin component.Because polyconazole is difficult to break down in soil and can negatively regulate the active chemicals in dahurica,excessive use of CCC or PP333 in dahurica agriculture has the potential to endanger both human health and the environment.To ensure the security and potency of Angelica dahurica herbs,it is advised that the primary locations for their production strictly regulate the usage of comparable PGR. |
PDF Full Text Request