Circular RNAs Profiles Of Osteoarthritic Synovium

Objective: To identify the Circular RNAs(circ RNAs)expression profile in the synovium of patients with Osteoarthritis(OA)and explore their potential regulatory mechanism.Methods: Transcriptome high-throughput sequencing was used to detect the expression profiles of circ RNA and m RNA.We performed quantitative Real-Time PCR for validation of circ RNAs and used bioinformatics analysis to predict their possible biological functions.The conservation of circ RNAs was evaluated,circ RNA-mi RNA-m RNA interaction network was constructed and the Receiver Operating Characteristic(ROC)curves of target genes were also drawn.Results: We found 136 differentially expressed circ RNAs,64 upregulated and 72 downregulated.We also found 2035 differentially expressed m RNAs,1216 upregulated and 819 downregulated.It was verified by q RT-PCR that hsa＿circ＿0072697 was significantly upregulated.GO analysis results showed that the parental genes were mainly enriched in organelle organization,cytosol and anion binding.KEGG results showed that the main enriched pathway of these circ RNAs was cell senescence.And hsa＿circ＿0072697 might act as a sponge of hsa-mi R-6736-5p,therefore could lead to increased LEP and ULK1 m RNA expression.Conclusion: Circ RNAs are significantly expressed in the knee synovium of OA patients,among which hsa＿circ＿0072697 has been verified to be significantly up-regulated in the OA knee joint synovium tissue.