The Promotion Of Hair Growth By Stem Cell Membrane-loaded Minoxidil Nanosomes

ObjectiveTo prepare human umbilical cord-derived mesenchymal stem cell membrane-loaded minoxidil nanoparticles and to investigate their effects on hair growth in C57BL/6J mice.MethodWe obtained umbilical cord-derived mesenchymal stem cell membrane-loaded minoxidil nanoparticles by freeze-thaw and differential centrifugation,evaluated the effect of hair growth using C57BL/6 mice,and detected the expression level of mRNA of vascular endothelial growth factor(VEGF)by immunohistochemical method for Ki67 and β-Catenin,and q-PCR method.Results1.Change of lipid-soluble minoxidil to water-soluble minoxidil after stem cell membrane-loaded minoxidil.2.Minoxidil is released slowly after the stem cell membrane is loaded with minoxidil,and the amount of minoxidil released at 24 h is about 38%.3.Stem cell membranes loaded with minoxidil increase drug transdermal properties.4.Stem cell membranes loaded with minoxidil can increase the concentration of drug accumulation in the skin.5.5% Stem cell membrane-loaded minoxidil nanosome solution promotes hair growth in C57BL/6 mice better than 5% minoxidil solution.6.5% Stem cell membrane-loaded minoxidil nanosome solution increases Ki67 and β-Catenin protein expression and increases VEGF mRNA expression.7.The 5% stem cell membrane-loaded minoxidil solution group better promoted hair growth,increased Ki67 and β-Catenin protein and VEGF mRNA expression in C57BL/6J mice.Conclusion1.Stem cell membrane-loaded minoxidil nanosomes with slow-release properties that increase minoxidil transdermal properties and increase the concentration of minoxidil accumulation in the skin.2.5% Stem cell membrane-loaded minoxidil nanosome solution promotes hair growth in C57BL/6J mice compared to 5% minoxidil solution.3.5% Stem cell membrane-loaded minoxidil nanosome solution promotes hair growth by increasing Ki67 and β-Catenin protein expression and increasing VEGF mRNA expression pathway.