Correlation Between Differential Expression Of THBS3 In Peripheral Blood And Acute Myocardial Infarction

Background and objectiveCoronary atherosclerosis heart disease(CAD)ranks first among the leading causes of death in developed countries,while acute myocardial infarction(AMI)is the most serious manifestation of coronary artery disease,it threatens human health seriously.According to the China Cardiovascular Health and Disease Report 2021,the incidence of cardiovascular disease in China continues to increase,which is the main cause of death among urban and rural residents.Although primary and secondary prevention has improved the treatment and prognosis of AMI,the risk of major adverse cardiovascular events remains high.Therefore,reducing the prevalence and mortality rate of the disease is a critical issue that needs to be solved urgently at this stage.In this case,this study was based on proteomic results to further validate the correlation between the differential expression of Thrombospondin 3(THBS3)in peripheral blood and AMI at the protein and m RNA levels by expanding the sample size.MethodsIn the study,112 patients with AMI and 111 patients with stable coronary artery disease(SCAD)were selected as the experimental group and control group,and their peripheral blood was collected.Peripheral blood plasma and mononuclear cells were obtained by centrifugation for enzyme-linked immunosorbent assay(ELISA)and real-time quantitative polymerase chain reaction(RT-q PCR).The expression of THBS3 at the protein and m RNA levels in the two groups was detected by the above experiments.At the same time,the clinical data of the study subjects were collected,baseline information was recorded,and SPSS was used for baseline data analysis,correlation analysis and logistic regression analysis.ResultsThe results showed that the expression of THBS3 in the AMI group was4.526(3.748-5.521)(ng/ml),the SCAD group was 5.511(4.726-6.334)(ng/ml)at the protein level in peripheral plasma,the expression level in the AMI group was lower than 82% in the control group(P<0.001).At the m RNA level,the expression of THBS3 in peripheral blood mononuclear cells in the AMI group was significantly lower than that of 47% in the control group(P<0.05).By collating the clinical data of the study subjects,it was found that compared with the control group,the AMI group had higher levels of total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),fasting blood glucose levels(P<0.001),older age(P<0.001),higher platelet count(PLT)levels(P<0.05),and a greater proportion of smoking history in the AMI group(P<0.01).However,there were no statistical differences in triglycerides,highdensity lipoprotein cholesterol,hypertension,and diabetes(P>0.05).Further correlation analysis showed that there was a correlation between low THBS3 expression and age and fasting blood glucose(P<0.01),but not with TC,LDL-C,PLT,and smoking history(P>0.05).Binary logistic regression analysis showed that low expression of THBS3 protein was an independent risk factor for AMI,and the risk of AMI increased by 4.076 times(P<0.01).In addition,high fasting blood glucose and high platelet count were also independent risk factors for the disease,and high fasting blood glucose increased the risk of AMI by 2.819 times(P<0.05),high platelet count increased the risk of AMI by 6.515 times(P<0.01).In the Receiver-operating characteristic curve(ROC)analysis,the area under the curve was 0.718(P<0.001),and the sensitivity and specificity for diagnosing AMI were 79.3% and 52.7%,respectively.Conclusion1.The expression of THBS3 in peripheral blood in the AMI group was decreased at the m RNA and protein levels.2.Low expression of THBS3 in peripheral blood is an independent risk factor for AMI.3.Low expression of THBS3 in peripheral blood may serve as a novel molecular marker for the acute onset of AMI.