Relationship Between ZMPSTE24 Expression Level And Glioma: A CGGA Database Study

Objective:To find the relationship between the ZMPSTE24’s lever and glioma^[1].Methods:The data is come form the Chinese Glioma Genome Atlas（CGGA）whice include Clinical data and m RNA sequencing data of 325 patients^[1].The ZMPSTE24’s lever was extracted and combined with clinical data,and the cases with missing clinical data were excluded^[1].According to the median expression of ZMPSTE24 in glioma patients（18.61）,these were divided into two groups^[1].One group is high expression and the other is low expression^[1].Wilcoxon signed-rank test and logistic regression were used to find the correlation,whice were ZMPSTE24 level with clinical factors^[1].The effect of ZMPSTE24expression level on overall survival of glioma patients was evaluated using Cox regression and Kaplan-Meier methods^[1].ZMPSTE24 associated molecular pathways and upstream and downstream molecules were estimated using gene enrichment and coexpression analysis^[1].The STRING database was used to find the molecules interacting with ZMPSTE24and to evaluate their value^[1].The TIM ER2.0 database was used to investigate the correlation between ZMPSTE24expression level and glioma immune infiltrating cells^[1].Finally,the Human Protein Atlas（HPA）and clinical glioma pathological tissues were used to perform immunohistochemical and Western blotting tests for verification^[1].Results:Compared with ZMPSTE24 group with high expression,patients with high expression of ZMPSTE24 had significantly worse prognosis（P<0.05）in CGGA database^[1].The glioma classification,IDH classification and age classification is relate with ZMPSTE24（P<0.05）^[1].Cox regression analysis showed that ZMPSTE24might be an independent prognostic factor for glioma patients（P<0.05）^[1].Western blot indicated that the higher the tumor grade,the higher the expression level of ZMPSTE24^[1].Conclusion:High expression of ZMPSTE24is associated with high tumor grade and poor p rognosis in glioma patients^[1].