Investigation Of The Relationship Between PrP~C Levels In Blood And Urine And Disease In Patients With Three Types Of Central Infectious Diseases

Objective:To detect the level changes of PrP~C in blood and urine in CNS infectious diseases such as viral meningoencephalitis,purulent meningitis and tuberculous meningoencephalitis,and to analyze its relationship with cerebrospinal fluid leukocyte count,blood neutrophil percentage and ultrasensitive C-reactive protein count,and to investigate the role of PrP~C in blood and urine in the early diagnosis,therapeutic efficacy and prognostic assessment.Methods:111 patients with CNS infectious diseases,27 with viral encephalitis(VE),16 with viral meningitis(VM),40 with purulent meningitis(PM),and 28 with tuberculous meningoencephalitis(TBM),were selected from January 2022 to December 2022 at the First Hospital of Jilin University as inpatients.The patients’basic information,auxiliary examination results and 5 ml of blood and 15 ml of urine were recorded,and the levels of PrP~C in the blood and urine of the three groups of patients were measured by ELISA.Statistical analysis was performed using SPSS statistical software,and indicators conforming to normal distribution were compared using independent sample t-test,while non-normal distribution was compared using U-test(Mann-Whitney)and H-test(Kruskall-Wallis).The X~2 test was used to compare the differences between groups for numerical data.Pearson or Spearman was used for correlation analysis.Statistical significance was considered at P<0.05.Results:1.Comparison of PrP~C levels in blood and urine of patients with disease in the viral meningoencephalitis group,the purulent meningitis group and the tuberculous meningoencephalitis group showed that the highest PrP~C levels were found in the blood of patients with purulent meningitis and the lowest in patients with tuberculous meningoencephalitis,but there was no statistical difference between the three.The PrP~Ccontent in urine of patients with viral meningoencephalitis was higher than that of patients with purulent meningitis and tuberculous meningoencephalitis,but it was not statistically significant.2.Blood PrP~C levels in patients with viral meningoencephalitis differed between patients with mild,moderate,and severe disease,and this difference was statistically significant(P<0.05).There was no statistical difference between patients with different severity of purulent meningitis and tuberculous meningoencephalitis.3.Blood PrP~C levels in patients with purulent meningitis increased during the exacerbation phase of the disease and decreased after the improvement of the disease,and the difference was statistically significant(P<0.05),while blood PrP~C levels in patients with tuberculous meningoencephalitis showed a continuous increase during the beginning,progression and remission phases of the disease,and the difference was statistically significant(P<0.05).4.There was a negative correlation between the blood PrP~C content and the percentage of lymphocytes in the blood of patients with viral meningoencephalitis,with a correlation coefficient of-0.368(P<0.05).There was a positive correlation between blood PrP~C content and blood neutrophil percentage and ultrasensitive C-reactive protein in patients with purulent meningitis,with correlation coefficients of 0.387 and0.363(P<0.05).5.Blood PrP~C levels in patients with viral meningoencephalitis differed among patients with mild,moderate,and severe disease,and this difference was statistically significant(P<0.05).There was no statistical difference between patients with different severity of purulent meningitis and tuberculous meningoencephalitis.Conclusion:1.PrP~C levels in the blood of patients with viral meningoencephalitis reflects the severity of the patients’disease.2.Blood PrP~C levels in patients with purulent meningitis and tuberculous meningoencephalitis can reflect the progress of disease and treatment effect.3.Blood PrP~C levels in patients with viral meningoencephalitis was negatively correlated with the percentage of lymphocytes in the blood,while PrP~C level in the blood of patients with purulent meningitis was positively correlated with the percentage of neutrophils and ultrasensitive C-reactive protein,suggesting that PrP~C level can reflect the degree of inflammatory response of patients with viral meningoencephalitis and purulent meningitis to a certain extent.