Exploring The Proteomic Differences Between Endometrial Atypical Hyperplasia And Endometrial Cancer

Objective This study is a series of liquid-phase chromatography mass spectrometry-based proteomic analyses to screen out significantly different proteins in normal endometrium,atypical hyperplastic endometrium,and endometrial cancer tissue,and to analyze their biological functions and mechanisms of action,so as to provide a basis for future exploration of the mechanisms of action in the development and progression of atypical endometrial hyperplasia to endometrial carcinoma and to explore new targets for the treatment of the disease.MethodsIn this study,21 cases of normal endometrium,5 cases of endometrial atypical hyperplasia and 5 cases of Endometrial cancer were collected from the Second Clinical Affiliated Hospital of Jinan University,and the proteins were identified and quantitative information was obtained by Label-free 4D proteomic quantification technique.The tissues were protein extracted and enzymatically cleaved into peptides and enriched.The proteins were detected and quantified by liquid chromatography-mass spectrometry(LC-MS/MS)analysis.Significantly differentially expressed proteins were screened from three groups of endosomal tissues,and these differentially expressed proteins were subjected to in-depth bioinformatics analysis by public database search.Results(1)The study was divided into three groups: 21 cases in the normal endometrial group,5 cases in the atypical endometrial hyperplasia group,and 6 cases in the endometrial cancer group.A total of 5880 proteomes were identified,and differential analysis was performed on the basis of "sample stable expression proteins".The six DEPs that were significantly upregulated in the atypical hyperplasia group and further upregulated in the endometrial cancer group were CDK1,TRAM1,TSPO,ACY1,HSP90AB4 P,USP14.(2)The development and progression of atypical hyperplasia to endometrial cancer was known to be associated with oxidative phosphorylation,oxidoreductase activity,and peroxidase activity from Gene Ontology.(3)The occurrence and progression of atypical hyperplasia to endometrial cancer was known to be associated with Phenylalanine metabolism and Oxidative phosphorylation pathways from Kyoto Encyclopedia of Genes and Genomes.ConclusionsThis study identified multiple proteins and pathways associated with the development of endometrial atypical hyperplasia and endometrial cancer through proteomic differential analysis,providing a researchable target for predicting new approaches to such diseases and future treatments.