The Expression Level Of Lysine Methyltransferase 2D In Bladder Cancer And Its Effect On Cell Proliferation And Invasion

Objective : To detect the relationship between the expression level of lysine methyltransferase 2D(KMT2D)in bladder cancer and clinicopathological characteristics and the effect of KMT2 D on the proliferation and invasion of bladder cancer cells.Methods:The KMT2 D protein and m RNA expression levels were determined in bladder cancer tissues and adjacent cancer tissues by immunohistochemistry experiments and q RT-PCR experiments,respectively.Patients were divided into KMT2 D low-expression and KMT2 D high-expression groups based on the immunohistochemistry score to compare the clinicopathological characteristics of the two groups.Western blotting was performed to detect the expression level of KMT2 D protein in Bladder cancer cells of 5637 、 T24 、 TCCSUP and Normal bladder epithelial cells of SV-HUC-1.The mi R-NC plasmid and mi R-KMT2 D plasmid were transfected into 5637,T24 and TCCSUP cells,Transfection efficiency was detected by protein immunoimprinting science experiments.Cells were examined for proliferation and invasion by MTT and transwell experiments after successful transfection.Results:The score of KMT2 D protein immunohistochemistry in bladder cancer tissue was(43.0±6.7)points lower than that of adjacent tissues(92.1±5.9),t=17.371,P<0.001.The relative expression level of KMT2 D m RNA in bladder cancer tissue was(0.8±0.2)lower than that in adjacent tissues(2.6±0.4),t=7.606,P=0.002;Compared with KMT2 D low and high expression groups,the difference between the age,the degree of tumor differentiation and the depth of invasive bladder,respectively(P<0.05);KMT2D protein expression levels in bladder cancer cells 5637,T24 and TCCSUP were(0.38 ± 0.03),(0.42 ± 0.03),(0.03)and(0.36 ± 0.05)were lower than SV-HUC-1(0.92 ± 0.06),q=21.131,19.584,21.647,all P <0.001;After mi R-NC plasmid and mi R-KMT2 D plasmid were transfected into 5637,T24 and TCCSUP cells,the KMT2 D protein level of 5637-KMT2 D 、 T24-KMT2 D 、 TCCSUPKMT2 D groups increased significantly;When the cells were cultured for 72 hours,the cell OD value of the 5637-KMT2 D group(0.73±0.05)was lower than that of the 5637-NC group(1.34±0.07)(t=8.241,P<0.001),and the cell OD value of the T24-KMT2 D group(0.67±0.06)lower than T24-NC group(1.12±0.06)(t=9.524,P<0.001),TCCSUP-KMT2 D group cell OD value(0.62±0.07)lower than TCCSUP-NC group(1.32±0.06)(t=8.941,P <0.001);The number of invaded cells in the 5637-KMT2 D group(34.5±4.7)was lower than that in the 5637-NC group(56.0±5.2)(t=3.524,P=0.008),and the number of invaded cells in the T24-KMT2 D group(36.4±4.5)was lower in the T24-NC group(68.5±6.7)(t=7.302,P<0.001),the number of invasive cells in the TCCSUP-KMT2 D group(30.1±3.4)was lower than that in the TCCSUPNC group(74.2±6.5)(t=8.206,P<0.001).Conclusion:The expression level of KMT2 D in bladder cancer is significantly lower than that of adjacent tissues,indicating that KMT2 D plays a role in regulating cell metabolism and proliferation;increasing the expression level of KMT2 D can weaken cell proliferation and invasion,and KMT2 D is expected to be an effective target for bladder cancer.