| Objective: To use metabolomics LC-MS technology to process the in-situ endometrium of EMT patients and non-EMT to screen out metabolites with obvious differences in tissues,search for the metabolic pathways involved in differential metabolites and organisms,and further explore the pathological mechanism of endometrium in patients with endometriosis.Methods: Ultra-high performance liquid chromatography(UHPLC)and Q extraction quadrupole orbital high-resolution mass spectrometer were applied to non-targeted metabolomics analysis,and non-targeted metabolomics analysis was performed on 21 EMT patients and 27 non-EMT patients,and the original peak data were obtained,and the original peak was further filtered,peak aligned,and normalized by correcting the retention time and extracting the peak area of the original peak.Metaboanalyst 5.0 software was used to perform multivariate statistical analysis of the obtained data,and the multivariate statistical analysis methods used included principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA),and it was found that the two sets of data had a clear separation trend through statistical analysis.Based on the variable weights(VIPs)obtained by t-test analysis and OPLS-DA on the data,metabolites with P<0.05 and VIP>1 were screened.Search for metabolic differences through metabolite databases such as KEGG and HMDB to obtain more information on metabolites.The screened differential metabolites were put into the Pathway Analysis section of Metaboanalyst 5.0 to search for metabolic pathways related to differential metabolites to further explore the pathological mechanisms associated with EMT.Results: After a variety of statistical analysis of the data,the results showed that there were 51 differential metabolites in the endometrium of ENT and non-EMT patients,of which a total of 11 differential metabolites could be searched in the existing database,including N-acetylglutamic acid,Formiminoglutamic acid,pantothenic acid,N-acetyl-L-aspartic acid and 5-hydroxyeicosenoic acid(5-HETE)were concentrated in the experimental group compared with the control group,and the concentration of Cholic acid,Taurocholic acid,Sphingosine,aminoadipic acid,4-The experimental group of 6 metabolites,acetamidobutanoic acid and uric acid,were lower than those in the control group.There are a total of 11 metabolic pathways related to this: Primary bile acid biosynthesis,Taurine and hypotaurine metabolism,Arginine biosynthesis,and Histidine metabolism),pantothenate and coenzyme A biosynthesis pathway(Pantothenate and Co A biosynthesis),Sphingolipid metabolism pathway(Sphingolipid metabolism),lysine degradation pathway(Lysine degradation),alanine,aspartate and glutamate metabolism pathway(Alanine,aspartate and glutamate metabolism),arachidonic acid metabolism,Arginine and proline metabolism,purine metabolism.Conclusion: There were statistically significant differences in endometrial metabolites between EMT group and non-EMT groups.It has been found that organisms may cause endometriosis by promoting inflammation,reducing antioxidant capacity,promoting angiogenesis,promoting cell migration,affecting cellular energy synthesis,destroying cells and affecting cell repair,but because the process of metabolic pathways related to this is not very complete in the current database,further experiments are needed to study the relevant pathological mechanism of EMT.Through this experiment,it was found that it is a good technical means to study diseases through metabolomics,but because of its limited technology and equipment,the cost is expensive and not yet popularized,and further development is needed to bring metabolomics technology into the clinic for the diagnosis and treatment of diseases. |
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