| research objectiveThrough the comparative analysis of high-intensity interval training(HIIT)and aerobic training on the indicators of blood sugar,blood fat,bone metabolism,inflammatory factors and related protein expression of type 2 diabetes rats after modeling,we found the most important factors affecting the bone metabolism of type 2 diabetes rats and the best exercise mode,and enriched the knowledge related to exercise on the prevention of type 2 diabetes,It also provides data support and training guidance for sports treatment of type 2 diabetes.Research methodsAfter one week of adaptive rearing,8-week-old female normal rats were randomly divided into two groups,normal control group(SC group,n=25)and type 2 diabetes modeling group(DS group,N=75),SC group was fed normally,DS group was injected with high fat and high sugar + STZ solution,fasting blood glucose and insulin levels were checked,rats with successful type 2 diabetes modeling were screened,and the type 2 diabetes model group was grouped,namely: modeling group + aerobic exercise group(CAMP group,n=21)and modeling group + High-intensity interval exercise group(HIIT group,n=21),CAMP group for 8 weeks of aerobic exercise(5 days a week,once a day,each time 1 hour 15m/min treadmill exercise),HIIT group for 8 weeks of high-intensity interval exercise(5 days a week,once a day,each time 12m/min exercise 14 min warm-up,then exercise at 18m/min for 5min,exercise at 42m/min for 4min,and then alternately in turn,repeat 4 times,Finally,relax at a speed of 10m/min for 10min).After 8 weeks of exercise intervention,the blood glucose,blood lipid and insulin levels of rats were detected by automatic biochemical analyzer.Bone density in rats was tested with a dual-energy X-ray bone density scanner;The levels of TNF-α,IL-6,and serum osteocalcin in rats were tested with ELISA kits;Rats IRS-1 and IRS-2 protein expression were determined by Westernblot.Research results1.Glucose and lipid metabolism in rats: Compared with the DS group,the SC group showed significant differences in body weight,blood sugar,insulin,TG,TC,and FFA(P<0.01),indicating that after feeding with a high fat and high sugar diet and injecting STZ solution,the DS group experienced an increase in body weight,blood sugar,insulin,and blood lipids.The modeling results in this article were very ideal.After 8 weeks of exercise intervention,compared with the DS group,only the HIIT group in the intervention group showed a very significant difference(P<0.01),while the CAMP group showed a downward trend without significant difference.Compared with the CAMP group,the HIIT group showed a significant difference in blood sugar(P<0.05),indicating that aerobic exercise is more beneficial for improving insulin levels during high-intensity intermittent exercise.After 8 weeks of exercise intervention,compared with the DS group,the TG,TC,and FFA of the HIIT and CAMP groups were significantly reduced(P<0.01),indicating that exercise intervention has a significant effect on reducing blood lipids in T2DM;At the same time,comparing the HIIT group with the CAMP group,it was found that there was a very significant difference in TC and FFA between the HIIT group(P<0.01),while there was no significant difference in TG.This indicates that in terms of reducing TC and FFA,the effect of high-intensity intermittent exercise intervention is better than aerobic exercise,and in terms of reducing TG,both interventions have the same effect.2.Bone metabolism and protein levels in rats: After 8 weeks of exercise intervention,compared with the DS group,the BGP levels in the HIIT and CAMP groups were significantly increased(P<0.01);By comparing two intervention methods,it was found that compared with the CAMP group,the HIIT group had a more significant increase in BGP(P<0.01),indicating that high-intensity intermittent exercise had a better effect on improving BGP;After 8 weeks of exercise intervention,compared with the DS group,the IRS-1 of the HIIT and CAMP groups was significantly reduced,while the IRS-2 of the HIIT and CAMP groups was increased,indicating the effectiveness of exercise intervention.Comparing two different types of exercise interventions simultaneously,it was found that compared with the CAMP group,the HIIT group had a more significant increase in IRS-2,with no significant change in IRS-1.This indicates that high-intensity intermittent exercise has a better effect on improving IRS-2 in T2 MD rats.3.Inflammatory factors in rats: After 8 weeks of exercise intervention,compared with the DS group,the serum leptin levels in the HIIT and CAMP groups were significantly increased(P<0.01),while IL-6 levels were significantly reduced(P<0.01),while TNF levels in both groups were significantly increased(P<0.01)-α Although there is a downward trend,there is no significant difference,indicating that the intervention of two types of exercise has an effect on serum leptin and IL-6,and has an impact on TNF-α The effect is not significant.At the same time,by comparing the intervention of two types of exercise,it was found that compared with CAMP group,the serum leptin and IL-6 in HIIT group were significantly different(P<0.01),indicating that compared with aerobic exercise,high-intensity intermittent exercise had a better intervention effect on inflammatory factors in diabetes rats.research conclusion1.After 8 weeks of high-intensity intermittent exercise and aerobic exercise,the metabolism of glucose and lipid in type 2 diabetes rats has a good improvement.2.After 8 weeks of high-intensity intermittent exercise and aerobic exercise,bone metabolism and inflammatory factors in type 2 diabetes rats can be improved.3.The key factor that high-intensity intermittent exercise and aerobic exercise affect bone metabolism and inflammatory factors in type 2 diabetes rats may be exercise intensity.4.Through the influence of high-intensity intermittent exercise and aerobic exercise on bone metabolism and inflammatory factors in type 2 diabetes rats,high-intensity intermittent exercise is superior to aerobic exercise. |
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